236386

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3994198..3994748 Replicon chromosome
Accession NZ_CP092532
Organism Escherichia coli strain DS566-2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag MKD45_RS19430 Protein ID WP_001160966.1
Coordinates 3994198..3994512 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag MKD45_RS19435 Protein ID WP_000125568.1
Coordinates 3994515..3994748 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD45_RS19405 (3989873) 3989873..3991159 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
MKD45_RS19410 (3991159) 3991159..3992148 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
MKD45_RS19415 (3992145) 3992145..3992966 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
MKD45_RS19420 (3992969) 3992969..3993346 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
MKD45_RS19425 (3993353) 3993353..3994201 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
MKD45_RS19430 (3994198) 3994198..3994512 - 315 WP_001160966.1 CcdB family protein Toxin
MKD45_RS19435 (3994515) 3994515..3994748 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MKD45_RS19440 (3994834) 3994834..3995313 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
MKD45_RS19445 (3995505) 3995505..3997367 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
MKD45_RS19450 (3997360) 3997360..3997890 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
MKD45_RS19455 (3997998) 3997998..3999329 - 1332 WP_001183207.1 MFS transporter -
MKD45_RS19460 (3999388) 3999388..3999675 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T236386 WP_001160966.1 NZ_CP092532:c3994512-3994198 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T236386 NZ_LR590469:c2011252-2010965 [Yersinia enterocolitica subsp. enterocolitica]
ATGACTTATATCGTTAAATTCAGAGACGAAGCGCTAAAGGAATGGCACAAACTGGATAAGACGATACAACAGCAGTTTGC
CAAGAAATTAAAAAAATGCAGTGAAAATCCCCATATTGAAAGTGCCAGACTGCGGGGGATCAAGAATGGTTACAAAATTA
AATTACGTGCCTCTGGTTTTCGCCTCGTGTATGAAGTTATTGATGGTATTTTGGTAGTTGCAGTGGTTGCGGTAGGGAAA
AGAGAACGTAGTGAGGTTTATAAACTGGCCAGTGATAGATTACGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT236386 WP_000125568.1 NZ_CP092532:c3994748-3994515 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT236386 NZ_LR590469:c2011500-2011249 [Yersinia enterocolitica subsp. enterocolitica]
ATGCCAAGTGTTATTTTGACAGATACCAGCGCGAGCGTAAGTGAACTGAAGAAAAATCCCATGGCGACAGTGAATGCTGG
GGCAGGTTTCCCTGTTGCTATTCTGAACCGTAATCAACCTGTATTTTATTGTGTGCCCGCTGAACTCTATGAGCAAATAA
TGGAAGCGCTTGATGATCATGAATTAGCCAGGCTGGTGAATGAACGTCGTAATCAACCACTTCATGATATTGATTTGGAT
AGCTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References