Detailed information of TA system TA023638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3083379..3083983
• Replicon: chromosome
• Accession: NC_012207
• Organism: Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: JTY_RS14630
• Protein ID: WP_003414492.1
• Coordinates: 3083379..3083771 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: JTY_RS14635
• Protein ID: WP_003414495.1
• Coordinates: 3083768..3083983 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY_RS14600	3078529..3079317	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
JTY_RS14605	3079651..3080196	-	546	WP_003904931.1	DUF1802 family protein	-
JTY_RS14610	3080468..3081352	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JTY_RS14615	3081355..3082242	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
JTY_RS14620	3082547..3083092	-	546	WP_003899500.1	DUF1802 family protein	-
JTY_RS14625	3083089..3083358	-	270	WP_003414489.1	DUF2277 family protein	-
JTY_RS14630	3083379..3083771	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
JTY_RS14635	3083768..3083983	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JTY_RS14640	3084030..3084779	+	750	WP_003414497.1	enoyl-CoA hydratase	-
JTY_RS14645	3084858..3085940	-	1083	WP_011799293.1	ABC transporter ATP-binding protein	-
JTY_RS21460	3085933..3087243	-	1311	Protein_2855	ABC transporter substrate-binding protein	-
JTY_RS14660	3087246..3088073	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
JTY_RS14665	3088070..3088981	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T23638 WP_003414492.1 NC_012207:c3083771-3083379 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T23638 NC_012207:c3083771-3083379 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT23638 WP_003414495.1 NC_012207:c3083983-3083768 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT23638 NC_012207:c3083983-3083768 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8