Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 933453..934107 Replicon chromosome
Accession NZ_CP092532
Organism Escherichia coli strain DS566-2

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag MKD45_RS04530 Protein ID WP_000244781.1
Coordinates 933700..934107 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MKD45_RS04525 Protein ID WP_000354046.1
Coordinates 933453..933719 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD45_RS04505 (929541) 929541..930974 - 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -
MKD45_RS04510 (931019) 931019..931330 + 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
MKD45_RS04515 (931494) 931494..932153 + 660 WP_000250274.1 hemolysin III family protein -
MKD45_RS04520 (932230) 932230..933210 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
MKD45_RS04525 (933453) 933453..933719 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MKD45_RS04530 (933700) 933700..934107 + 408 WP_000244781.1 protein YgfX Toxin
MKD45_RS04535 (934147) 934147..934668 - 522 WP_001055874.1 flavodoxin FldB -
MKD45_RS04540 (934780) 934780..935676 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MKD45_RS04545 (935701) 935701..936411 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MKD45_RS04550 (936417) 936417..938150 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T236371 WP_000244781.1 NZ_CP092532:933700-934107 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T236371 NZ_LR588412:3918587-3918805 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT236371 WP_000354046.1 NZ_CP092532:933453-933719 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT236371 NZ_LR588412:3918186-3918560 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References