Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 82341..83077 Replicon plasmid p1
Accession NZ_CP092513
Organism Klebsiella quasipneumoniae strain EF262

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MKM89_RS26150 Protein ID WP_003026803.1
Coordinates 82595..83077 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MKM89_RS26145 Protein ID WP_003026799.1
Coordinates 82341..82607 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKM89_RS26120 (MKM89_26115) 77807..78880 + 1074 WP_017900354.1 urea ABC transporter permease subunit UrtC -
MKM89_RS26125 (MKM89_26120) 78880..79677 + 798 WP_004205978.1 urea ABC transporter ATP-binding protein UrtD -
MKM89_RS26130 (MKM89_26125) 79687..80385 + 699 WP_004205977.1 urea ABC transporter ATP-binding subunit UrtE -
MKM89_RS26135 (MKM89_26130) 80421..80903 - 483 WP_004205976.1 helix-turn-helix domain-containing protein -
MKM89_RS26140 (MKM89_26135) 80963..81931 + 969 WP_072202995.1 IS5 family transposase -
MKM89_RS26145 (MKM89_26140) 82341..82607 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MKM89_RS26150 (MKM89_26145) 82595..83077 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MKM89_RS26155 (MKM89_26150) 83289..84635 + 1347 WP_077251107.1 ISNCY family transposase -
MKM89_RS26160 (MKM89_26155) 84795..85499 + 705 WP_031591821.1 toll/interleukin-1 receptor domain-containing protein -
MKM89_RS26165 (MKM89_26160) 85834..86091 - 258 WP_023292103.1 hypothetical protein -
MKM89_RS26170 (MKM89_26165) 86734..87249 + 516 Protein_94 CSS-motif domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..203351 203351
- inside IScluster/Tn - - 80963..84635 3672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T236366 WP_003026803.1 NZ_CP092513:82595-83077 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T236366 NZ_LR588412:355076-355423 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT236366 WP_003026799.1 NZ_CP092513:82341-82607 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT236366 NZ_LR588412:355423-355722 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References