Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 16024..16667 Replicon plasmid p1
Accession NZ_CP092513
Organism Klebsiella quasipneumoniae strain EF262

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag MKM89_RS25780 Protein ID WP_021312475.1
Coordinates 16251..16667 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A6B7Q5S4
Locus tag MKM89_RS25775 Protein ID WP_021312476.1
Coordinates 16024..16254 (+) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKM89_RS25750 (MKM89_25745) 11532..12047 + 516 Protein_10 CSS-motif domain-containing protein -
MKM89_RS25755 (MKM89_25750) 12147..12947 + 801 WP_065905270.1 EAL domain-containing protein -
MKM89_RS25760 (MKM89_25755) 13634..14602 - 969 WP_014839937.1 IS5 family transposase -
MKM89_RS25765 (MKM89_25760) 14604..15077 - 474 WP_065905236.1 hypothetical protein -
MKM89_RS25770 (MKM89_25765) 15126..15419 - 294 WP_124989603.1 hypothetical protein -
MKM89_RS25775 (MKM89_25770) 16024..16254 + 231 WP_021312476.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MKM89_RS25780 (MKM89_25775) 16251..16667 + 417 WP_021312475.1 type II toxin-antitoxin system VapC family toxin Toxin
MKM89_RS25785 (MKM89_25780) 16702..16947 + 246 WP_065905238.1 hypothetical protein -
MKM89_RS25790 (MKM89_25785) 17011..17229 + 219 WP_021312527.1 hypothetical protein -
MKM89_RS25795 (MKM89_25790) 17308..17634 + 327 WP_124989605.1 hypothetical protein -
MKM89_RS25800 (MKM89_25795) 17874..18137 + 264 WP_004118691.1 hypothetical protein -
MKM89_RS25805 (MKM89_25800) 18134..18700 + 567 WP_009654312.1 hypothetical protein -
MKM89_RS25810 (MKM89_25805) 18731..19225 + 495 WP_016528789.1 hypothetical protein -
MKM89_RS25815 (MKM89_25810) 19286..19489 + 204 WP_004150739.1 HHA domain-containing protein -
MKM89_RS25820 (MKM89_25815) 19538..19795 + 258 WP_004098928.1 hypothetical protein -
MKM89_RS25825 (MKM89_25820) 19871..20125 + 255 WP_032731922.1 hypothetical protein -
MKM89_RS25830 (MKM89_25825) 20424..21428 - 1005 WP_065905242.1 IS110-like element IS5075 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..203351 203351
- flank IS/Tn - - 13634..14602 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15033.44 Da        Isoelectric Point: 7.8915

>T236365 WP_021312475.1 NZ_CP092513:16251-16667 [Klebsiella quasipneumoniae]
VKKTFMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Download         Length: 417 bp

>T236365 NZ_LR588412:329106-329474 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTTATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8723.76 Da        Isoelectric Point: 4.3410

>AT236365 WP_021312476.1 NZ_CP092513:16024-16254 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Download         Length: 231 bp

>AT236365 NZ_LR588412:328888-329109 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGAAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A6B7Q5S4

References