Detailed information of TA system TA236362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2582466..2584054
• Replicon: chromosome
• Accession: NZ_CP092512
• Organism: Klebsiella quasipneumoniae strain EF262

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: MKM89_RS12570
• Protein ID: WP_048324183.1
• Coordinates: 2582466..2583788 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: MKM89_RS12575
• Protein ID: WP_004205921.1
• Coordinates: 2583788..2584054 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKM89_RS12535 (2577548)	2577548..2577766	+	219	WP_002904808.1	ParD-like family protein	-
MKM89_RS12540 (2577747)	2577747..2578529	+	783	WP_049003659.1	type I methionyl aminopeptidase	-
MKM89_RS12545 (2578798)	2578798..2579913	+	1116	WP_249107777.1	serine hydrolase domain-containing protein	-
MKM89_RS12550 (2579900)	2579900..2580244	+	345	WP_004205915.1	hypothetical protein	-
MKM89_RS12555 (2580273)	2580273..2580806	+	534	WP_004205916.1	hypothetical protein	-
MKM89_RS12560 (2580809)	2580809..2581015	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
MKM89_RS12565 (2581157)	2581157..2582449	+	1293	WP_048324186.1	glycoside hydrolase family 10 protein	-
MKM89_RS12570 (2582466)	2582466..2583788	-	1323	WP_048324183.1	type II toxin-antitoxin system HipA family toxin	Toxin
MKM89_RS12575 (2583788)	2583788..2584054	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MKM89_RS12580 (2584337)	2584337..2584663	-	327	WP_017900379.1	hydroxyisourate hydrolase	-
MKM89_RS12585 (2584660)	2584660..2585160	-	501	WP_048324181.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
MKM89_RS12590 (2585138)	2585138..2586517	-	1380	WP_249107779.1	nucleobase:cation symporter-2 family protein	-
MKM89_RS12595 (2586863)	2586863..2588017	+	1155	WP_032456029.1	FAD-dependent urate hydroxylase HpxO	-
MKM89_RS12600 (2587998)	2587998..2588921	-	924	WP_048324174.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48899.16 Da
• Isoelectric Point: 7.8920

>T236362 WP_048324183.1 NZ_CP092512:c2583788-2582466 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARQTLPSDFSAHVWGAITENMLKLHARLQQGLLAG

Nucleotide

• Length: 1323 bp

>T236362 NZ_LR588411:c2889229-2889011 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT236362 WP_004205921.1 NZ_CP092512:c2584054-2583788 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT236362 NZ_LR588411:c2889630-2889256 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9