23636

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3056388..3056958 Replicon chromosome
Accession NC_012207
Organism Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JTY_RS14480 Protein ID WP_003414166.1
Coordinates 3056388..3056744 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JTY_RS14485 Protein ID WP_003901465.1
Coordinates 3056728..3056958 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTY_RS14460 3051840..3053528 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
JTY_RS14465 3053532..3053858 - 327 WP_003414157.1 hypothetical protein -
JTY_RS14470 3054031..3054618 + 588 WP_003914429.1 DUF3558 family protein -
JTY_RS14475 3054637..3056286 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
JTY_RS14480 3056388..3056744 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JTY_RS14485 3056728..3056958 - 231 WP_003901465.1 antitoxin MazE Antitoxin
JTY_RS14490 3057001..3058044 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
JTY_RS21995 3058235..3058510 + 276 WP_003911993.1 DUF1778 domain-containing protein -
JTY_RS22000 3058686..3058940 - 255 WP_003917684.1 hypothetical protein -
JTY_RS14505 3059088..3059492 + 405 WP_003414181.1 hypothetical protein -
JTY_RS14510 3059489..3059680 + 192 WP_003414184.1 hypothetical protein -
JTY_RS14515 3059744..3061033 + 1290 Protein_2829 transposase family protein -
JTY_RS14520 3061267..3061524 + 258 WP_003899489.1 hypothetical protein -
JTY_RS14525 3061629..3061940 + 312 WP_010950777.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-113)

Antitoxin

(3-29)


Sequences


Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T23636 WP_003414166.1 NC_012207:c3056744-3056388 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T23636 NC_012207:c3056744-3056388 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT23636 WP_003901465.1 NC_012207:c3056958-3056728 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT23636 NC_012207:c3056958-3056728 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS


Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FBW3

References