Detailed information of TA system TA023635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3016805..3017492
• Replicon: chromosome
• Accession: NC_012207
• Organism: Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: JTY_RS14260
• Protein ID: WP_003414064.1
• Coordinates: 3017097..3017492 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JTY_RS14255
• Protein ID: WP_003414061.1
• Coordinates: 3016805..3017071 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY_RS14230	3012444..3013346	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JTY_RS14235	3013415..3014167	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JTY_RS14240	3014411..3014686	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JTY_RS14245	3014683..3016305	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
JTY_RS14250	3016392..3016808	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
JTY_RS14255	3016805..3017071	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JTY_RS14260	3017097..3017492	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
JTY_RS14265	3017489..3017758	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JTY_RS14270	3017768..3018862	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JTY_RS14275	3018859..3019278	-	420	Protein_2782	winged helix-turn-helix domain-containing protein	-
JTY_RS14280	3019352..3019831	-	480	WP_003414073.1	dihydrofolate reductase	-
JTY_RS14285	3019902..3020702	-	801	WP_003911953.1	thymidylate synthase	-
JTY_RS14290	3020858..3021595	+	738	WP_011799288.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T23635 WP_003414064.1 NC_012207:c3017492-3017097 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T23635 NC_012207:c3017492-3017097 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT23635 WP_003414061.1 NC_012207:c3017071-3016805 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT23635 NC_012207:c3017071-3016805 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64