Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 2744..3345 Replicon plasmid pEV38
Accession NZ_CP092506
Organism Lactiplantibacillus plantarum strain VHProbi V38

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag MKM38_RS15100 Protein ID WP_001748110.1
Coordinates 2744..3088 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag MKM38_RS15105 Protein ID WP_001748109.1
Coordinates 3082..3345 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKM38_RS15095 (MKM38_15095) 90..2387 - 2298 WP_249262113.1 DUF1906 domain-containing protein -
MKM38_RS15100 (MKM38_15100) 2744..3088 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MKM38_RS15105 (MKM38_15105) 3082..3345 - 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MKM38_RS15110 (MKM38_15110) 3431..4018 + 588 WP_063490192.1 site-specific integrase -
MKM38_RS15115 (MKM38_15115) 4381..4593 + 213 WP_001748064.1 hypothetical protein -
MKM38_RS15120 (MKM38_15120) 5313..6248 + 936 WP_001748065.1 replication initiation protein -
MKM38_RS15125 (MKM38_15125) 6241..6759 + 519 WP_001748066.1 HTH domain-containing protein -
MKM38_RS15130 (MKM38_15130) 6973..7062 + 90 Protein_7 IS30 family transposase -
MKM38_RS15135 (MKM38_15135) 7104..7571 - 468 WP_003587210.1 DNA starvation/stationary phase protection protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..8624 8624


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T236329 WP_001748110.1 NZ_CP092506:c3088-2744 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T236329 NZ_LR536431:928414-928821 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT236329 WP_001748109.1 NZ_CP092506:c3345-3082 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT236329 NZ_LR536431:928167-928433 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References