Detailed information of TA system TA236326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3802349..3802925
• Replicon: chromosome
• Accession: NZ_CP092500
• Organism: Escherichia coli strain A30

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: LU695_RS18975
• Protein ID: WP_174561634.1
• Coordinates: 3802638..3802925 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A7U9DAW4
• Locus tag: LU695_RS18970
• Protein ID: WP_000063150.1
• Coordinates: 3802349..3802651 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU695_RS18950 (3797959)	3797959..3798471	+	513	WP_001175455.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-
LU695_RS18955 (3798864)	3798864..3801014	+	2151	WP_001298933.1	pyruvate/proton symporter BtsT	-
LU695_RS18960 (3801145)	3801145..3801348	+	204	WP_000467859.1	YbdD/YjiX family protein	-
LU695_RS18965 (3801359)	3801359..3802315	+	957	WP_077492876.1	GTPase	-
LU695_RS18970 (3802349)	3802349..3802651	-	303	WP_000063150.1	BrnA antitoxin family protein	Antitoxin
LU695_RS18975 (3802638)	3802638..3802925	-	288	WP_174561634.1	BrnT family toxin	Toxin
LU695_RS18980 (3803081)	3803081..3803764	-	684	WP_258313996.1	cell filamentation protein Fic	-
LU695_RS18985 (3803843)	3803843..3804540	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
LU695_RS18990 (3804556)	3804556..3804831	+	276	Protein_3723	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
LU695_RS18995 (3805073)	3805073..3806029	-	957	WP_258313997.1	Rpn family recombination-promoting nuclease/putative transposase	-
LU695_RS19000 (3806496)	3806496..3807728	+	1233	WP_174561682.1	multidrug efflux MFS transporter MdtM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3804037..3804540	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11212.68 Da
• Isoelectric Point: 6.8811

>T236326 WP_174561634.1 NZ_CP092500:c3802925-3802638 [Escherichia coli]
MPMEFEWDANKAKSNWVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T236326 NZ_LR536431:339541-339816 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTCCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11532.03 Da
• Isoelectric Point: 9.4646

>AT236326 WP_000063150.1 NZ_CP092500:c3802651-3802349 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT236326 NZ_LR536431:339813-340172 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTATGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DAW4