Detailed information of TA system TA236325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3339894..3340731
• Replicon: chromosome
• Accession: NZ_CP092500
• Organism: Escherichia coli strain A30

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: LU695_RS16690
• Protein ID: WP_000227784.1
• Coordinates: 3340189..3340731 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: LU695_RS16685
• Protein ID: WP_001297137.1
• Coordinates: 3339894..3340205 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU695_RS16660 (3334914)	3334914..3335861	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
LU695_RS16665 (3335883)	3335883..3337874	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
LU695_RS16670 (3337864)	3337864..3338478	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
LU695_RS16675 (3338478)	3338478..3338807	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
LU695_RS16680 (3338819)	3338819..3339709	+	891	WP_000971336.1	heme o synthase	-
LU695_RS16685 (3339894)	3339894..3340205	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
LU695_RS16690 (3340189)	3340189..3340731	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
LU695_RS16695 (3340787)	3340787..3341254	-	468	Protein_3274	hypothetical protein	-
LU695_RS16705 (3342513)	3342513..3342983	-	471	Protein_3276	tetratricopeptide repeat protein	-
LU695_RS16710 (3343391)	3343391..3344755	+	1365	WP_032314626.1	MFS transporter	-
LU695_RS16715 (3344883)	3344883..3345374	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T236325 WP_000227784.1 NZ_CP092500:3340189-3340731 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T236325 NZ_LR536431:c56056-55715 [Escherichia coli]
ATGAAACCTCAATCTGCAACAATTTCGCGGGCGGCGAAGCCTTGCCTGTCGCCCGTGGCAGTCTGGCAAATGCTGCTGAT
CCGACTGCTCGAAAAGCACTATGGCCTGACGCTAAACGACACCCCGTTCAGTGATGAAACTGTGATTCAGGAACACATTA
ATGCCGGGATCACTCTGGCCGATGCTGTGAATTTTCTGGTGGAGAAATACGAACTAGTGCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCCCCTTATCTTCGGGCTGTCGATATTCTGCGAGCGCGACAGGCAACTGGCCTGTTGCGACGAAA
TCGAAAACACACTGCATTGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT236325 WP_001297137.1 NZ_CP092500:3339894-3340205 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT236325 NZ_LR536431:c56394-56077 [Escherichia coli]
ATGAGCAATATCACATGGGGCCTGCAGCGGGATATCACGCCGCGTCTGGGAGTCCGTCTGGTGCAGGAGGGCAACCGGCT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACCCCGAATGCCGGAAGCTGGATGAAACTTTCCCGC
ACTTTATCAGCCAGATGGAATTAATGCTGACTACCGGTGAACTGAATCCCCGCCATACCCACTGCATTGCCCTGTACCAC
AATGGTTTTACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGCTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3