Detailed information of TA system TA236312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1215662..1216287
• Replicon: chromosome
• Accession: NZ_CP092500
• Organism: Escherichia coli strain A30

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LU695_RS05990
• Protein ID: WP_000911330.1
• Coordinates: 1215889..1216287 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LU695_RS05985
• Protein ID: WP_000450524.1
• Coordinates: 1215662..1215889 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU695_RS05960 (1211466)	1211466..1211936	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
LU695_RS05965 (1211936)	1211936..1212508	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LU695_RS05970 (1212654)	1212654..1213532	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LU695_RS05975 (1213549)	1213549..1214583	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
LU695_RS05980 (1214795)	1214795..1215508	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LU695_RS05985 (1215662)	1215662..1215889	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LU695_RS05990 (1215889)	1215889..1216287	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
LU695_RS05995 (1216434)	1216434..1217297	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
LU695_RS06000 (1217312)	1217312..1219327	+	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
LU695_RS06005 (1219401)	1219401..1220099	+	699	WP_000679812.1	esterase	-
LU695_RS06010 (1220209)	1220209..1220409	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T236312 WP_000911330.1 NZ_CP092500:1215889-1216287 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T236312 NZ_LR536430:c1777594-1777253 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTAGCACCCGTGGCTGTCTGGCAAATGCTACTGAC
CCGCCTGCTGAAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGAATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTCGGTACGGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT236312 WP_000450524.1 NZ_CP092500:1215662-1215889 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT236312 NZ_LR536430:c1777932-1777615 [Escherichia coli]
ATGACCCACATCAACTGGGGCCTACAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGGGTAACCGACT
GCACTATCTGGCTGACAGGGCCAGCATCACCGGCAGGTTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATGCCCGC
ACTTTATCCGCCAGATGGAGTCGATGCTAACCACCGGTGAACTCAGTCCCCAACACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGAGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33