Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 833372..834032 Replicon chromosome
Accession NZ_CP092500
Organism Escherichia coli strain A30

Toxin (Protein)


Gene name cptA Uniprot ID A0A3W5XVE6
Locus tag LU695_RS04120 Protein ID WP_000244778.1
Coordinates 833619..834032 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LU695_RS04115 Protein ID WP_000354046.1
Coordinates 833372..833638 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU695_RS04090 (828660) 828660..829403 + 744 WP_258314122.1 SDR family oxidoreductase -
LU695_RS04095 (829460) 829460..830893 - 1434 WP_001343615.1 6-phospho-beta-glucosidase BglA -
LU695_RS04100 (830938) 830938..831249 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
LU695_RS04105 (831413) 831413..832072 + 660 WP_000250274.1 hemolysin III family protein -
LU695_RS04110 (832149) 832149..833129 - 981 WP_258314123.1 tRNA-modifying protein YgfZ -
LU695_RS04115 (833372) 833372..833638 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LU695_RS04120 (833619) 833619..834032 + 414 WP_000244778.1 protein YgfX Toxin
LU695_RS04125 (834066) 834066..834587 - 522 WP_001055874.1 flavodoxin FldB -
LU695_RS04130 (834699) 834699..835595 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LU695_RS04135 (835619) 835619..836329 + 711 WP_258314124.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LU695_RS04140 (836335) 836335..838068 + 1734 WP_258314125.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16275.23 Da        Isoelectric Point: 11.7064

>T236309 WP_000244778.1 NZ_CP092500:833619-834032 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

>T236309 NZ_LR536430:c1476356-1476021 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAAGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT236309 WP_000354046.1 NZ_CP092500:833372-833638 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT236309 NZ_LR536430:c1476604-1476356 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5XVE6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References