Detailed information of TA system TA236306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1267182..1268099
• Replicon: chromosome
• Accession: NZ_CP092499
• Organism: Bacillus velezensis strain YC_89

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: MJ920_RS06660
• Protein ID: WP_025851786.1
• Coordinates: 1267353..1268099 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: MJ920_RS06655
• Protein ID: WP_003154807.1
• Coordinates: 1267182..1267352 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ920_RS06615 (MJ920_06630)	1262413..1264035	+	1623	WP_278269543.1	pyocin knob domain-containing protein	-
MJ920_RS06620 (MJ920_06635)	1264048..1264419	+	372	WP_014304856.1	XkdW family protein	-
MJ920_RS06625 (MJ920_06640)	1264425..1264622	+	198	WP_003154819.1	XkdX family protein	-
MJ920_RS06630 (MJ920_06645)	1264679..1265440	+	762	WP_043867020.1	hypothetical protein	-
MJ920_RS06635 (MJ920_06650)	1265492..1265755	+	264	WP_003154815.1	hemolysin XhlA family protein	-
MJ920_RS06640 (MJ920_06655)	1265769..1266032	+	264	WP_003154813.1	phage holin	-
MJ920_RS06645 (MJ920_06660)	1266046..1266924	+	879	WP_025851788.1	N-acetylmuramoyl-L-alanine amidase	-
MJ920_RS06650 (MJ920_06665)	1266960..1267085	-	126	WP_003154809.1	hypothetical protein	-
MJ920_RS06655 (MJ920_06670)	1267182..1267352	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
MJ920_RS06660 (MJ920_06675)	1267353..1268099	-	747	WP_025851786.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MJ920_RS06665 (MJ920_06680)	1268204..1269202	-	999	WP_003154805.1	inorganic phosphate transporter	-
MJ920_RS06670 (MJ920_06685)	1269215..1269832	-	618	WP_003154804.1	DUF47 domain-containing protein	-
MJ920_RS06675 (MJ920_06690)	1270118..1271434	-	1317	WP_003154801.1	amino acid permease	-
MJ920_RS06680 (MJ920_06695)	1271757..1272707	+	951	WP_015388352.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29048.53 Da
• Isoelectric Point: 4.7003

>T236306 WP_025851786.1 NZ_CP092499:c1268099-1267353 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CEYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T236306 NZ_LR536430:c472310-470988 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT236306 WP_003154807.1 NZ_CP092499:c1267352-1267182 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT236306 NZ_LR536430:c472576-472310 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14