Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 476926..477563 | Replicon | chromosome |
| Accession | NZ_CP092499 | ||
| Organism | Bacillus velezensis strain YC_89 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | MJ920_RS02430 | Protein ID | WP_003156187.1 |
| Coordinates | 477213..477563 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | MJ920_RS02425 | Protein ID | WP_003156188.1 |
| Coordinates | 476926..477207 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MJ920_RS02405 (MJ920_02410) | 473291..473890 | - | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
| MJ920_RS02410 (MJ920_02415) | 473983..474348 | + | 366 | WP_014304402.1 | holo-ACP synthase | - |
| MJ920_RS02415 (MJ920_02420) | 474513..475520 | + | 1008 | WP_003156191.1 | outer membrane lipoprotein carrier protein LolA | - |
| MJ920_RS02420 (MJ920_02425) | 475637..476806 | + | 1170 | WP_003156189.1 | alanine racemase | - |
| MJ920_RS02425 (MJ920_02430) | 476926..477207 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| MJ920_RS02430 (MJ920_02435) | 477213..477563 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| MJ920_RS02435 (MJ920_02440) | 477681..478502 | + | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| MJ920_RS02440 (MJ920_02445) | 478507..478872 | + | 366 | WP_003156180.1 | RsbT antagonist protein RsbS | - |
| MJ920_RS02445 (MJ920_02450) | 478875..479276 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| MJ920_RS02450 (MJ920_02455) | 479288..480295 | + | 1008 | WP_003156177.1 | PP2C family protein-serine/threonine phosphatase | - |
| MJ920_RS02455 (MJ920_02460) | 480359..480688 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| MJ920_RS02460 (MJ920_02465) | 480685..481167 | + | 483 | WP_003156173.1 | anti-sigma B factor RsbW | - |
| MJ920_RS02465 (MJ920_02470) | 481133..481921 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| MJ920_RS02470 (MJ920_02475) | 481921..482523 | + | 603 | WP_003156170.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T236305 WP_003156187.1 NZ_CP092499:477213-477563 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T236305 NZ_LR536430:334544-334720 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT236305 WP_003156188.1 NZ_CP092499:476926-477207 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT236305 NZ_LR536430:334766-335182 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|