Detailed information of TA system TA236305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 476926..477563
• Replicon: chromosome
• Accession: NZ_CP092499
• Organism: Bacillus velezensis strain YC_89

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: MJ920_RS02430
• Protein ID: WP_003156187.1
• Coordinates: 477213..477563 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: MJ920_RS02425
• Protein ID: WP_003156188.1
• Coordinates: 476926..477207 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ920_RS02405 (MJ920_02410)	473291..473890	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
MJ920_RS02410 (MJ920_02415)	473983..474348	+	366	WP_014304402.1	holo-ACP synthase	-
MJ920_RS02415 (MJ920_02420)	474513..475520	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
MJ920_RS02420 (MJ920_02425)	475637..476806	+	1170	WP_003156189.1	alanine racemase	-
MJ920_RS02425 (MJ920_02430)	476926..477207	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
MJ920_RS02430 (MJ920_02435)	477213..477563	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MJ920_RS02435 (MJ920_02440)	477681..478502	+	822	WP_003156182.1	STAS domain-containing protein	-
MJ920_RS02440 (MJ920_02445)	478507..478872	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
MJ920_RS02445 (MJ920_02450)	478875..479276	+	402	WP_003156178.1	anti-sigma regulatory factor	-
MJ920_RS02450 (MJ920_02455)	479288..480295	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
MJ920_RS02455 (MJ920_02460)	480359..480688	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
MJ920_RS02460 (MJ920_02465)	480685..481167	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
MJ920_RS02465 (MJ920_02470)	481133..481921	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
MJ920_RS02470 (MJ920_02475)	481921..482523	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T236305 WP_003156187.1 NZ_CP092499:477213-477563 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T236305 NZ_LR536430:334544-334720 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT236305 WP_003156188.1 NZ_CP092499:476926-477207 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT236305 NZ_LR536430:334766-335182 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5