Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 645753..646404 Replicon chromosome
Accession NZ_CP092498
Organism Lacticaseibacillus paracasei strain VHProbi OF10

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag MKL87_RS03150 Protein ID WP_003567661.1
Coordinates 646021..646404 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag MKL87_RS03145 Protein ID WP_003567665.1
Coordinates 645753..646001 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKL87_RS03120 (MKL87_03110) 640823..642211 + 1389 WP_144182137.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
MKL87_RS03125 (MKL87_03115) 642242..642385 + 144 WP_016380391.1 hypothetical protein -
MKL87_RS03130 (MKL87_03120) 642511..644019 + 1509 WP_003567670.1 DEAD/DEAH box helicase -
MKL87_RS03135 (MKL87_03125) 644188..644562 + 375 WP_003567669.1 holo-ACP synthase -
MKL87_RS03140 (MKL87_03130) 644549..645685 + 1137 WP_003581082.1 alanine racemase -
MKL87_RS03145 (MKL87_03135) 645753..646001 + 249 WP_003567665.1 antitoxin Antitoxin
MKL87_RS03150 (MKL87_03140) 646021..646404 + 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MKL87_RS03155 (MKL87_03145) 646588..647109 + 522 WP_003567657.1 QueT transporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 612582..656731 44149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T236303 WP_003567661.1 NZ_CP092498:646021-646404 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T236303 NZ_LR536430:c54582-54475 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT236303 WP_003567665.1 NZ_CP092498:645753-646001 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT236303 NZ_LR536430:54629-54695 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References