Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 140010..140813 Replicon plasmid pOXA1_SCLZS47
Accession NZ_CP092496
Organism Citrobacter freundii strain SCLZS47

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag MJ253_RS26745 Protein ID WP_022652313.1
Coordinates 140283..140813 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag MJ253_RS26740 Protein ID WP_022652312.1
Coordinates 140010..140279 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ253_RS26725 (MJ253_26725) 136315..136622 - 308 Protein_150 IS1-like element transposase -
MJ253_RS26730 (MJ253_26730) 137099..137839 + 741 WP_022652310.1 site-specific integrase -
MJ253_RS26735 (MJ253_26735) 138165..139154 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
MJ253_RS26740 (MJ253_26740) 140010..140279 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
MJ253_RS26745 (MJ253_26745) 140283..140813 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
MJ253_RS26750 (MJ253_26750) 140981..141838 + 858 WP_022652314.1 IS5 family transposase -
MJ253_RS26755 (MJ253_26755) 141979..142872 - 894 Protein_156 IS3 family transposase -
MJ253_RS26760 (MJ253_26760) 142940..144091 + 1152 WP_022652315.1 IS30-like element IS30 family transposase -
MJ253_RS26765 (MJ253_26765) 144580..144927 + 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / mph(A) / blaTEM-1B / aac(3)-IId / tet(D) / sul2 / qnrA1 / aadA2 / dfrA12 - 1..209894 209894
- inside IScluster/Tn - - 135001..148382 13381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T236302 WP_022652313.1 NZ_CP092496:140283-140813 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T236302 NZ_LR536430:c54047-53940 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGGACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT236302 WP_022652312.1 NZ_CP092496:140010-140279 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT236302 NZ_LR536430:54095-54161 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References