Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 66831..67474 Replicon plasmid pOXA1_SCLZS47
Accession NZ_CP092496
Organism Citrobacter freundii strain SCLZS47

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NE46
Locus tag MJ253_RS26350 Protein ID WP_008322233.1
Coordinates 67058..67474 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag MJ253_RS26345 Protein ID WP_001261276.1
Coordinates 66831..67061 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ253_RS26330 (MJ253_26330) 64151..64711 - 561 WP_003124096.1 recombinase family protein -
MJ253_RS26335 (MJ253_26335) 64766..65470 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
MJ253_RS26340 (MJ253_26340) 65556..66578 + 1023 WP_008322227.1 helicase UvrD -
MJ253_RS26345 (MJ253_26345) 66831..67061 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MJ253_RS26350 (MJ253_26350) 67058..67474 + 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin Toxin
MJ253_RS26355 (MJ253_26355) 68020..68940 - 921 WP_008322235.1 carbohydrate kinase -
MJ253_RS26360 (MJ253_26360) 68937..70307 - 1371 WP_008322237.1 hypothetical protein -
MJ253_RS26365 (MJ253_26365) 70385..71404 - 1020 WP_004113181.1 ribose ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / mph(A) / blaTEM-1B / aac(3)-IId / tet(D) / sul2 / qnrA1 / aadA2 / dfrA12 - 1..209894 209894


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15054.58 Da        Isoelectric Point: 9.2957

>T236301 WP_008322233.1 NZ_CP092496:67058-67474 [Citrobacter freundii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAASAVLVTNNVREFARVPGLMLEDWVK

Download         Length: 417 bp

>T236301 NZ_LR535679:c1063120-1062797 [Campylobacter showae]
TTGTGGGAAATTACATTTTTAGATGACCGAGTAGCTGAAGAATTTAAAGCCCTACCTCCTAAAATTTCCGCTAACATGAC
CCATATATTTAAGCTCTTACGTCTTTACGGCAATCAAGTCGGTGAGCCGCAAACTAAAAGCCTAGGCGACGGGCTTTTTG
AGATAAGGGCTAAGGGCGCGGAGGGTATAGGCAGAGGAATATTTTGCTATATGACCGGTAGGCGCATTTATATATTGCAC
GTGTTTGTAAAGAAAGGTGACAGGATACCTCAAAAAGAGCTAGATATCGCCAAAAATAGATTAAAGGAGTTAAAAAATGC
CTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT236301 WP_001261276.1 NZ_CP092496:66831-67061 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT236301 NZ_LR535679:c1062804-1062523 [Campylobacter showae]
ATGCCTGAAGTAAAATTTGACGACGTATTTAATGAGATGATGAAAAATGATGAATTTAGAGCTGAATACGAAGCCTTGAT
GCCTGAATACGAACTAAAAAGCGAGTTGATAAAAGCTAGAATAAAAAGCGGACTGACGCAGTCGCAGCTAGCGGAGAGGA
TGGGTATGAAACAGTCCAATCTAGCTAGACTAGAAAGCGCAAGCGGAGATTTTAAATTTCAGACAATAATCAAATACGCA
AAAGCATTGGGACTAAAGCAGCTAAATATCGCACTGAACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE46

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References