Detailed information of TA system TA236300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 41244..41755
• Replicon: plasmid pKPC2_SCLZS47
• Accession: NZ_CP092494
• Organism: Citrobacter freundii strain SCLZS47

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W0NIW1
• Locus tag: MJ253_RS25560
• Protein ID: WP_020956903.1
• Coordinates: 41244..41519 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MJ253_RS25565
• Protein ID: WP_020956904.1
• Coordinates: 41531..41755 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ253_RS25530 (MJ253_25530)	37280..37567	-	288	WP_020956899.1	TrbC/VirB2 family protein	-
MJ253_RS25535 (MJ253_25535)	37554..38192	-	639	WP_020956900.1	transglycosylase SLT domain-containing protein	-
MJ253_RS25540 (MJ253_25540)	38406..38738	+	333	WP_227551116.1	helix-turn-helix transcriptional regulator	-
MJ253_RS25545 (MJ253_25545)	38768..39958	-	1191	WP_041937886.1	MobP1 family relaxase	-
MJ253_RS25550 (MJ253_25550)	39960..40514	-	555	WP_020956901.1	DNA distortion polypeptide 1	-
MJ253_RS25555 (MJ253_25555)	40956..41222	-	267	WP_020956902.1	hypothetical protein	-
MJ253_RS25560 (MJ253_25560)	41244..41519	-	276	WP_020956903.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
MJ253_RS25565 (MJ253_25565)	41531..41755	-	225	WP_020956904.1	DUF6290 family protein	Antitoxin
MJ253_RS25570 (MJ253_25570)	42067..42318	-	252	WP_240210407.1	hypothetical protein	-
MJ253_RS25575 (MJ253_25575)	42429..42575	-	147	WP_240210408.1	hypothetical protein	-
MJ253_RS25580 (MJ253_25580)	43004..43237	-	234	WP_240210409.1	hypothetical protein	-
MJ253_RS25585 (MJ253_25585)	43242..43463	-	222	WP_020956907.1	helix-turn-helix domain-containing protein	-
MJ253_RS25590 (MJ253_25590)	43460..43705	-	246	WP_020956908.1	hypothetical protein	-
MJ253_RS25595 (MJ253_25595)	43702..44244	-	543	WP_020956909.1	hypothetical protein	-
MJ253_RS25600 (MJ253_25600)	44335..44859	-	525	WP_020956910.1	DnaJ domain-containing protein	-
MJ253_RS25605 (MJ253_25605)	44967..45326	+	360	WP_020956911.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2	-	1..45886	45886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-88)

Antitoxin

• Protein of unknown function DUF6290 (6-72)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10922.82 Da
• Isoelectric Point: 10.6500

>T236300 WP_020956903.1 NZ_CP092494:c41519-41244 [Citrobacter freundii]
MTRYRTEYTKRFVKEFKKIDTVHQRVIQKYIEAHINNALNPRLTGKILKGDKAEFIRWRIGDYRLIATIEDDKLLVIALS
VGHRKDVYKKF

Nucleotide

• Length: 276 bp

>T236300 NZ_LR217818:c4634106-4633933 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8535.52 Da
• Isoelectric Point: 4.3313

>AT236300 WP_020956904.1 NZ_CP092494:c41755-41531 [Citrobacter freundii]
MNKNMTKAIRLNDEDFNLFESYANAKGITFSELCKSAVREKIEDELDLQCANESYADYLSDKTTISHDELFKSM

Nucleotide

• Length: 225 bp

>AT236300 NZ_LR217818:c4634430-4634134 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A384ZT31