Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4946431..4947047 Replicon chromosome
Accession NZ_CP092493
Organism Citrobacter freundii strain SCLZS47

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag MJ253_RS24495 Protein ID WP_003028682.1
Coordinates 4946431..4946805 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag MJ253_RS24500 Protein ID WP_043018956.1
Coordinates 4946805..4947047 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ253_RS24480 (4943934) 4943934..4944836 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
MJ253_RS24485 (4944833) 4944833..4945468 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
MJ253_RS24490 (4945465) 4945465..4946394 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
MJ253_RS24495 (4946431) 4946431..4946805 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
MJ253_RS24500 (4946805) 4946805..4947047 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
MJ253_RS24505 (4947253) 4947253..4948161 + 909 WP_003847901.1 alpha/beta hydrolase -
MJ253_RS24510 (4948312) 4948312..4949253 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
MJ253_RS24515 (4949298) 4949298..4949735 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
MJ253_RS24520 (4949732) 4949732..4950604 - 873 WP_003028669.1 virulence factor BrkB family protein -
MJ253_RS24525 (4950598) 4950598..4951197 - 600 WP_003028663.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T236299 WP_003028682.1 NZ_CP092493:c4946805-4946431 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T236299 NZ_LR217818:4622607-4622984 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGTTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTGCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT236299 WP_043018956.1 NZ_CP092493:c4947047-4946805 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT236299 NZ_LR217818:4622149-4622517 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCAGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References