Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 938721..939375 Replicon chromosome
Accession NZ_CP092493
Organism Citrobacter freundii strain SCLZS47

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag MJ253_RS04655 Protein ID WP_003026936.1
Coordinates 938968..939375 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag MJ253_RS04650 Protein ID WP_003026938.1
Coordinates 938721..938987 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ253_RS04625 (933922) 933922..935355 - 1434 WP_003838263.1 6-phospho-beta-glucosidase BglA -
MJ253_RS04630 (935476) 935476..936204 - 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
MJ253_RS04635 (936257) 936257..936568 + 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
MJ253_RS04640 (936731) 936731..937390 + 660 WP_003838267.1 hemolysin III family protein -
MJ253_RS04645 (937484) 937484..938464 - 981 WP_003838269.1 tRNA-modifying protein YgfZ -
MJ253_RS04650 (938721) 938721..938987 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
MJ253_RS04655 (938968) 938968..939375 + 408 WP_003026936.1 protein YgfX Toxin
MJ253_RS04660 (939420) 939420..939941 - 522 WP_003026933.1 flavodoxin FldB -
MJ253_RS04665 (940055) 940055..940951 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
MJ253_RS04670 (940975) 940975..941688 + 714 WP_003825520.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MJ253_RS04675 (941694) 941694..943427 + 1734 WP_016150943.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T236281 WP_003026936.1 NZ_CP092493:938968-939375 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T236281 NZ_LR217818:c1308873-1308595 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT236281 WP_003026938.1 NZ_CP092493:938721-938987 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT236281 NZ_LR217818:c1309136-1308876 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References