Detailed information of TA system TA236279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 373802..374388
• Replicon: chromosome
• Accession: NZ_CP092493
• Organism: Citrobacter freundii strain SCLZS47

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A0J1QS94
• Locus tag: MJ253_RS01810
• Protein ID: WP_032937237.1
• Coordinates: 374020..374388 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0J1N092
• Locus tag: MJ253_RS01805
• Protein ID: WP_032937236.1
• Coordinates: 373802..374023 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ253_RS01780 (369601)	369601..370527	+	927	WP_003023435.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
MJ253_RS01785 (370524)	370524..371801	+	1278	WP_003023436.1	branched chain amino acid ABC transporter permease LivM	-
MJ253_RS01790 (371798)	371798..372565	+	768	WP_003023438.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
MJ253_RS01795 (372583)	372583..373296	+	714	WP_003827581.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
MJ253_RS01800 (373399)	373399..373680	+	282	WP_003023442.1	hypothetical protein	-
MJ253_RS01805 (373802)	373802..374023	+	222	WP_032937236.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MJ253_RS01810 (374020)	374020..374388	+	369	WP_032937237.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MJ253_RS01815 (374632)	374632..375948	+	1317	WP_003837839.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
MJ253_RS01820 (376049)	376049..376936	+	888	WP_003837841.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
MJ253_RS01825 (376933)	376933..377778	+	846	WP_003023450.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
MJ253_RS01830 (377781)	377781..378851	+	1071	WP_003837845.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	370524..379591	9067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13617.91 Da
• Isoelectric Point: 6.9891

>T236279 WP_032937237.1 NZ_CP092493:374020-374388 [Citrobacter freundii]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIVLSANPDFVAMTVEAAAGQLTLEQIAHRLRH

Nucleotide

• Length: 369 bp

>T236279 NZ_LR217818:959291-959698 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8306.41 Da
• Isoelectric Point: 4.7906

>AT236279 WP_032937236.1 NZ_CP092493:373802-374023 [Citrobacter freundii]
MRTMNYSEARQNLAEVLESAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT236279 NZ_LR217818:959044-959310 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0J1QS94

Antitoxin

• AlphaFold DB: A0A0J1N092