236278

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 348960..349591 Replicon chromosome
Accession NZ_CP092493
Organism Citrobacter freundii strain SCLZS47

Toxin (Protein)


Gene name hicA Uniprot ID A0A0J1MPQ6
Locus tag MJ253_RS01660 Protein ID WP_003837806.1
Coordinates 348960..349235 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0J1MMS0
Locus tag MJ253_RS01665 Protein ID WP_003837808.1
Coordinates 349232..349591 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ253_RS01650 (345011) 345011..347758 + 2748 WP_032937231.1 ribosome-associated ATPase/putative transporter RbbA -
MJ253_RS01655 (347758) 347758..348882 + 1125 WP_003837801.1 ABC transporter permease -
MJ253_RS01660 (348960) 348960..349235 + 276 WP_003837806.1 type II toxin-antitoxin system HicA family toxin Toxin
MJ253_RS01665 (349232) 349232..349591 + 360 WP_003837808.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MJ253_RS01670 (349702) 349702..350103 - 402 WP_003023387.1 nickel-responsive transcriptional regulator NikR -
MJ253_RS01675 (350153) 350153..350731 - 579 WP_003837810.1 4'-phosphopantetheinyl transferase AcpT -
MJ253_RS01680 (350794) 350794..351843 - 1050 WP_003023393.1 AI-2E family transporter -
MJ253_RS01685 (351977) 351977..353194 + 1218 WP_032950436.1 MFS transporter -
MJ253_RS01690 (353198) 353198..353755 - 558 WP_003837816.1 DcrB family lipoprotein -
MJ253_RS01695 (353828) 353828..354493 - 666 WP_003837818.1 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10210.88 Da        Isoelectric Point: 10.7228

>T236278 WP_003837806.1 NZ_CP092493:348960-349235 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Download         Length: 276 bp

>T236278 NZ_LR217818:c830358-829984 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACTGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13198.98 Da        Isoelectric Point: 4.5462

>AT236278 WP_003837808.1 NZ_CP092493:349232-349591 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Download         Length: 360 bp

>AT236278 NZ_LR217818:c830779-830405 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGATGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MPQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MMS0

References