Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 33551..34194 Replicon plasmid unnamed1
Accession NZ_CP092467
Organism Citrobacter portucalensis strain Cf7303

Toxin (Protein)


Gene name vagD Uniprot ID A0A6C0NE46
Locus tag LY264_RS24825 Protein ID WP_008322233.1
Coordinates 33551..33967 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LY264_RS24830 Protein ID WP_001261276.1
Coordinates 33964..34194 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LY264_RS24810 (LY264_24805) 29621..30640 + 1020 WP_004113181.1 ribose ABC transporter permease -
LY264_RS24815 (LY264_24810) 30718..32088 + 1371 WP_008322237.1 hypothetical protein -
LY264_RS24820 (LY264_24815) 32085..33005 + 921 WP_008322235.1 carbohydrate kinase -
LY264_RS24825 (LY264_24820) 33551..33967 - 417 WP_008322233.1 type II toxin-antitoxin system VapC family toxin Toxin
LY264_RS24830 (LY264_24825) 33964..34194 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LY264_RS24835 (LY264_24830) 34447..35469 - 1023 WP_008322227.1 helicase UvrD -
LY264_RS24840 (LY264_24835) 35555..36259 + 705 WP_001067848.1 IS6-like element IS26 family transposase -
LY264_RS24845 (LY264_24840) 36662..37018 + 357 WP_000215515.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / catA2 / aac(3)-IId / blaTEM-1B / tet(D) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-1 / mph(A) / fosA3 / blaCTX-M-14 - 1..233864 233864


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15054.58 Da        Isoelectric Point: 9.2957

>T236237 WP_008322233.1 NZ_CP092467:c33967-33551 [Citrobacter portucalensis]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAASAVLVTNNVREFARVPGLMLEDWVK

Download         Length: 417 bp

>T236237 NZ_LR213458:c3063385-3063131 [Shigella sonnei]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT236237 WP_001261276.1 NZ_CP092467:c34194-33964 [Citrobacter portucalensis]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT236237 NZ_LR213458:c3063633-3063382 [Shigella sonnei]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE46

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References