Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 1846..2649 Replicon plasmid unnamed1
Accession NZ_CP092467
Organism Citrobacter portucalensis strain Cf7303

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag LY264_RS24695 Protein ID WP_022652313.1
Coordinates 2119..2649 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag LY264_RS24690 Protein ID WP_022652312.1
Coordinates 1846..2115 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LY264_RS24685 (LY264_24680) 1..990 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
LY264_RS24690 (LY264_24685) 1846..2115 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
LY264_RS24695 (LY264_24690) 2119..2649 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
LY264_RS24700 (LY264_24695) 2817..3674 + 858 WP_022652314.1 IS5 family transposase -
LY264_RS24705 (LY264_24700) 3815..4708 - 894 Protein_4 IS3 family transposase -
LY264_RS24710 (LY264_24705) 4776..5927 + 1152 WP_022652315.1 IS30-like element IS30 family transposase -
LY264_RS24715 (LY264_24710) 6416..6763 + 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / catA2 / aac(3)-IId / blaTEM-1B / tet(D) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-1 / mph(A) / fosA3 / blaCTX-M-14 - 1..233864 233864


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T236236 WP_022652313.1 NZ_CP092467:2119-2649 [Citrobacter portucalensis]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T236236 NZ_LR213458:2948116-2948223 [Shigella sonnei]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT236236 WP_022652312.1 NZ_CP092467:1846-2115 [Citrobacter portucalensis]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT236236 NZ_LR213458:c2948066-2948003 [Shigella sonnei]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References