Detailed information of TA system TA236233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4661773..4662308
• Replicon: chromosome
• Accession: NZ_CP092466
• Organism: Citrobacter portucalensis strain Cf7303

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LY264_RS22555
• Protein ID: WP_048227467.1
• Coordinates: 4662021..4662308 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D4BKM9
• Locus tag: LY264_RS22550
• Protein ID: WP_006688247.1
• Coordinates: 4661773..4662024 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY264_RS22520 (LY264_22515)	4656858..4657616	+	759	WP_048227463.1	phosphonate C-P lyase system protein PhnK	-
LY264_RS22525 (LY264_22520)	4658130..4658816	+	687	WP_048227464.1	phosphonate C-P lyase system protein PhnL	-
LY264_RS22530 (LY264_22525)	4658813..4659949	+	1137	WP_048227465.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
LY264_RS22535 (LY264_22530)	4659952..4660506	+	555	WP_048215112.1	ribose 1,5-bisphosphokinase	-
LY264_RS22540 (LY264_22535)	4660493..4660927	+	435	WP_048215113.1	aminoalkylphosphonate N-acetyltransferase	-
LY264_RS22545 (LY264_22540)	4660936..4661694	+	759	WP_048227466.1	phosphonate metabolism protein PhnP	-
LY264_RS22550 (LY264_22545)	4661773..4662024	+	252	WP_006688247.1	plasmid stabilization protein	Antitoxin
LY264_RS22555 (LY264_22550)	4662021..4662308	+	288	WP_048227467.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LY264_RS22560 (LY264_22555)	4662305..4662634	-	330	WP_003031807.1	DDRRRQL repeat protein YjdP	-
LY264_RS22565 (LY264_22560)	4662704..4664968	-	2265	WP_048227468.1	hybrid sensor histidine kinase/response regulator	-
LY264_RS22570 (LY264_22565)	4665083..4666606	+	1524	WP_003844755.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11165.14 Da
• Isoelectric Point: 10.3527

>T236233 WP_048227467.1 NZ_CP092466:4662021-4662308 [Citrobacter portucalensis]
MSYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCENPHIPSAKLRGIKDCYKIKLRTSGFRLVYQVIDDTLVIVVVAVGK
RERSEVYNLASERFR

Nucleotide

• Length: 288 bp

>T236233 NZ_LR213458:c2619589-2619299 [Shigella sonnei]
ATGTTACCCGTTTTATGGCTACCATCTGCTCGTGATGATTTGCGCCAGATCGTAGCCTACATAGCTAAAGAAAATCCCGC
GGCAGCACGCAGAATGAAAATTCGAATTGAAACATCCGTTTTGCCGCTCACAGAGCATCCTTACCTGTATCCTCCAAGCG
AGCGAGTTCTTGGTCTTCGTGAAATTGTAACTCATCCTAACTACATCATCCTTTACCGAGTAACAGCATCAAGCATAGAA
GTAGTAAATATTGTGCATTCTCGAAGACAGTATCCAGGCAAAAACAGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9215.53 Da
• Isoelectric Point: 4.2965

>AT236233 WP_006688247.1 NZ_CP092466:4661773-4662024 [Citrobacter portucalensis]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQQLVKLVHERSQQPLVDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT236233 NZ_LR213458:c2619783-2619589 [Shigella sonnei]
ATGAGTACAGTCCTTTCCCCAATGGTTTCAGAATTTGAGACAGTTGAGCAAGAAAATAGTTACAACGAATGGCTGCGGGC
CAAAGTAGCAGCAAGCCTCGCGGATCCCCGCCCGGCGATTCCGCATGATGAGGTAATGGCGGAAATGGAACAAATTATTG
CCCAAATTGCTGCTGCAACTCACAGAGGTGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7X1BT32