Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3780174..3780794 Replicon chromosome
Accession NZ_CP092466
Organism Citrobacter portucalensis strain Cf7303

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LY264_RS18355 Protein ID WP_002892050.1
Coordinates 3780576..3780794 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag LY264_RS18350 Protein ID WP_003021733.1
Coordinates 3780174..3780548 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LY264_RS18340 (LY264_18335) 3775320..3776513 + 1194 WP_048226274.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LY264_RS18345 (LY264_18340) 3776536..3779685 + 3150 WP_008783964.1 efflux RND transporter permease AcrB -
LY264_RS18350 (LY264_18345) 3780174..3780548 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
LY264_RS18355 (LY264_18350) 3780576..3780794 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LY264_RS18360 (LY264_18355) 3780976..3781527 + 552 WP_003835924.1 maltose O-acetyltransferase -
LY264_RS18365 (LY264_18360) 3781644..3782114 + 471 WP_020996574.1 YlaC family protein -
LY264_RS18370 (LY264_18365) 3782193..3782333 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
LY264_RS18375 (LY264_18370) 3782335..3782595 - 261 WP_008783961.1 type B 50S ribosomal protein L31 -
LY264_RS18380 (LY264_18375) 3782784..3784337 + 1554 WP_071692491.1 EAL domain-containing protein -
LY264_RS18385 (LY264_18380) 3784389..3784742 - 354 WP_048226278.1 DUF1428 family protein -
LY264_RS18390 (LY264_18385) 3784807..3785436 - 630 WP_048226280.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T236231 WP_002892050.1 NZ_CP092466:3780576-3780794 [Citrobacter portucalensis]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T236231 NZ_LR213458:c1475534-1475316 [Shigella sonnei]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT236231 WP_003021733.1 NZ_CP092466:3780174-3780548 [Citrobacter portucalensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT236231 NZ_LR213458:c1475934-1475560 [Shigella sonnei]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGAGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References