Detailed information of TA system TA023623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2229478..2230104
• Replicon: chromosome
• Accession: NC_012207
• Organism: Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JTY_RS10400
• Protein ID: WP_003410075.1
• Coordinates: 2229706..2230104 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JTY_RS10395
• Protein ID: WP_019283586.1
• Coordinates: 2229478..2229705 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY_RS10385	2227517..2227861	-	345	WP_003410065.1	ferredoxin family protein	-
JTY_RS10390	2228050..2229219	-	1170	WP_003899126.1	ATP-binding protein	-
JTY_RS10395	2229478..2229705	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JTY_RS10400	2229706..2230104	+	399	WP_003410075.1	PIN domain nuclease	Toxin
JTY_RS10405	2230287..2230718	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
JTY_RS10410	2230819..2231253	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
JTY_RS21175	2231690..2231869	-	180	Protein_2033	hypothetical protein	-
JTY_RS10415	2231930..2232574	+	645	WP_078396697.1	transposase	-
JTY_RS10420	2232528..2233118	+	591	WP_003899131.1	IS110 family transposase	-
JTY_RS10425	2233246..2234502	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T23623 WP_003410075.1 NC_012207:2229706-2230104 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T23623 NC_012207:2229706-2230104 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT23623 WP_019283586.1 NC_012207:2229478-2229705 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT23623 NC_012207:2229478-2229705 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG