Detailed information of TA system TA236229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2651739..2652475
• Replicon: chromosome
• Accession: NZ_CP092466
• Organism: Citrobacter portucalensis strain Cf7303

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A5B0TAB3
• Locus tag: LY264_RS12870
• Protein ID: WP_048214201.1
• Coordinates: 2651993..2652475 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LY264_RS12865
• Protein ID: WP_008784769.1
• Coordinates: 2651739..2652005 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY264_RS12845 (LY264_12845)	2646813..2647989	+	1177	Protein_2523	outer membrane protein transport protein	-
LY264_RS12850 (LY264_12850)	2648227..2648856	+	630	WP_071692397.1	winged helix-turn-helix transcriptional regulator	-
LY264_RS12855 (LY264_12855)	2648973..2649359	-	387	WP_048226505.1	hypothetical protein	-
LY264_RS12860 (LY264_12860)	2649603..2651585	+	1983	WP_071692396.1	alkyl sulfatase dimerization domain-containing protein	-
LY264_RS12865 (LY264_12865)	2651739..2652005	+	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
LY264_RS12870 (LY264_12870)	2651993..2652475	+	483	WP_048214201.1	GNAT family N-acetyltransferase	Toxin
LY264_RS12875 (LY264_12875)	2652825..2653346	-	522	WP_048226507.1	GNAT family N-acetyltransferase	-
LY264_RS12880 (LY264_12880)	2653457..2654215	+	759	WP_048226509.1	trans-aconitate 2-methyltransferase	-
LY264_RS12885 (LY264_12885)	2654347..2654724	+	378	WP_048226511.1	GFA family protein	-
LY264_RS12890 (LY264_12890)	2654721..2655635	-	915	WP_032943428.1	bestrophin family protein	-
LY264_RS12895 (LY264_12895)	2655851..2657302	-	1452	WP_032943427.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17572.35 Da
• Isoelectric Point: 9.1059

>T236229 WP_048214201.1 NZ_CP092466:2651993-2652475 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGATRTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHVLTDEAKRFYLHHGFKASQLQERTLFLGLS

Nucleotide

• Length: 483 bp

>T236229 NZ_LR213458:c1301560-1301282 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT236229 WP_008784769.1 NZ_CP092466:2651739-2652005 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT236229 NZ_LR213458:c1301823-1301563 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A5B0TAB3