Detailed information of TA system TA023622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2205738..2206324
• Replicon: chromosome
• Accession: NC_012207
• Organism: Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLY3
• Locus tag: JTY_RS10295
• Protein ID: WP_003410010.1
• Coordinates: 2205738..2206082 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL58
• Locus tag: JTY_RS10300
• Protein ID: WP_003410014.1
• Coordinates: 2206076..2206324 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTY_RS10260	2201444..2202043	+	600	WP_003409989.1	L-lysine exporter	-
JTY_RS10265	2202459..2202887	+	429	WP_003409992.1	cellulose-binding protein	-
JTY_RS10270	2203113..2203652	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
JTY_RS10275	2204172..2204732	-	561	WP_003410001.1	RES family NAD+ phosphorylase	-
JTY_RS10280	2204729..2205070	-	342	WP_003410003.1	DUF2384 domain-containing protein	-
JTY_RS10285	2205156..2205413	+	258	WP_003410006.1	hypothetical protein	-
JTY_RS10290	2205314..2205649	-	336	WP_003410009.1	dehydrogenase	-
JTY_RS10295	2205738..2206082	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	Toxin
JTY_RS10300	2206076..2206324	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	Antitoxin
JTY_RS10305	2206424..2208739	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
JTY_RS10310	2208736..2209008	-	273	WP_003410017.1	DUF1490 family protein	-
JTY_RS10315	2209061..2209417	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-
JTY_RS10320	2209574..2210341	+	768	WP_003410019.1	hemerythrin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-38)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12230.12 Da
• Isoelectric Point: 9.8887

>T23622 WP_003410010.1 NC_012207:c2206082-2205738 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Nucleotide

• Length: 345 bp

>T23622 NC_012207:c2206082-2205738 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9290.34 Da
• Isoelectric Point: 4.3757

>AT23622 WP_003410014.1 NC_012207:c2206324-2206076 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Nucleotide

• Length: 249 bp

>AT23622 NC_012207:c2206324-2206076 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Toxin

• PDB: 5HK3
• PDB: 5HK0
• PDB: 5HKC

Antitoxin

• PDB: 7WJ0
• PDB: 7WNR
• AlphaFold DB: A0A7U4FAW9