Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1061208..1061829 Replicon chromosome
Accession NZ_CP092461
Organism Serratia marcescens strain RH10

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag MJ023_RS05040 Protein ID WP_004940313.1
Coordinates 1061208..1061411 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag MJ023_RS05045 Protein ID WP_004940312.1
Coordinates 1061461..1061829 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ023_RS05010 (MJ023_001000) 1056908..1057246 + 339 WP_004940327.1 P-II family nitrogen regulator -
MJ023_RS05015 (MJ023_001001) 1057282..1058568 + 1287 WP_004940322.1 ammonium transporter AmtB -
MJ023_RS05020 (MJ023_001002) 1058659..1059522 - 864 WP_016928847.1 acyl-CoA thioesterase II -
MJ023_RS05025 (MJ023_001003) 1059753..1060244 + 492 WP_016928846.1 YbaY family lipoprotein -
MJ023_RS05030 (MJ023_001004) 1060309..1060623 - 315 WP_004940317.1 MGMT family protein -
MJ023_RS05040 (MJ023_001006) 1061208..1061411 - 204 WP_004940313.1 HHA domain-containing protein Toxin
MJ023_RS05045 (MJ023_001007) 1061461..1061829 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
MJ023_RS05050 (MJ023_001008) 1061988..1062341 - 354 WP_048795900.1 hypothetical protein -
MJ023_RS05055 (MJ023_001009) 1062746..1063459 + 714 WP_115116167.1 ABC transporter ATP-binding protein -
MJ023_RS05060 (MJ023_001010) 1063456..1064312 + 857 Protein_968 metal ABC transporter permease -
MJ023_RS05065 (MJ023_001011) 1064338..1065216 + 879 WP_016928843.1 metal ABC transporter substrate-binding protein -
MJ023_RS05070 (MJ023_001012) 1065322..1065462 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
MJ023_RS05075 (MJ023_001013) 1065475..1065729 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T236204 WP_004940313.1 NZ_CP092461:c1061411-1061208 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T236204 NZ_LR213452:3354818-3355036 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT236204 WP_004940312.1 NZ_CP092461:c1061829-1061461 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT236204 NZ_LR213452:3354418-3354792 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References