Detailed information of TA system TA236189

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 737181..737860
• Replicon: chromosome
• Accession: NZ_CP092460
• Organism: Dickeya fangzhongdai strain AP6

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: R8WLP2
• Locus tag: FXN80_RS03440
• Protein ID: WP_006812012.1
• Coordinates: 737519..737860 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: FXN80_RS03435
• Protein ID: WP_006812013.1
• Coordinates: 737181..737498 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXN80_RS03390 (FXN80_03390)	732613..733434	+	822	WP_000197389.1	DUF932 domain-containing protein	-
FXN80_RS03395 (FXN80_03395)	733651..734352	+	702	WP_006812588.1	WYL domain-containing protein	-
FXN80_RS03400 (FXN80_03400)	734393..734629	+	237	WP_001144031.1	protein YpjK	-
FXN80_RS03405 (FXN80_03405)	734629..735072	+	444	WP_001547764.1	lipoprotein YafY	-
FXN80_RS03410 (FXN80_03410)	735095..735562	+	468	WP_063841896.1	protein YkfB	-
FXN80_RS03415 (FXN80_03415)	735639..735878	+	240	WP_112020496.1	DUF905 domain-containing protein	-
FXN80_RS03420 (FXN80_03420)	735981..736439	+	459	WP_112020495.1	antirestriction protein	-
FXN80_RS03425 (FXN80_03425)	736455..736931	+	477	WP_047355118.1	RadC family protein	-
FXN80_RS03430 (FXN80_03430)	736940..737161	+	222	WP_000691995.1	DUF987 domain-containing protein	-
FXN80_RS03435 (FXN80_03435)	737181..737498	+	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FXN80_RS03440 (FXN80_03440)	737519..737860	+	342	WP_006812012.1	TA system toxin CbtA family protein	Toxin
FXN80_RS03450 (FXN80_03450)	738361..740196	-	1836	WP_049854589.1	RNA polymerase sigma factor RpoD	-
FXN80_RS03455 (FXN80_03455)	740353..742107	-	1755	WP_161130373.1	DNA primase	-
FXN80_RS03460 (FXN80_03460)	742235..742450	-	216	WP_001144069.1	30S ribosomal protein S21	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	732613..756955	24342

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12989.07 Da
• Isoelectric Point: 10.4476

>T236189 WP_006812012.1 NZ_CP092460:737519-737860 [Dickeya fangzhongdai]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T236189 NZ_LR135782:427481-427822 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT236189 WP_006812013.1 NZ_CP092460:737181-737498 [Dickeya fangzhongdai]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT236189 NZ_LR135782:427251-427481 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: R8WLP2

Antitoxin

• AlphaFold DB: R8WMM2