Detailed information of TA system TA236178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 1813627..1814380
• Replicon: chromosome
• Accession: NZ_CP092458
• Organism: Dickeya fangzhongdai strain 643b

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: MJO48_RS08125
• Protein ID: WP_107759008.1
• Coordinates: 1813627..1813884 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: A0A2K8QKC0
• Locus tag: MJO48_RS08130
• Protein ID: WP_038918520.1
• Coordinates: 1813892..1814380 (+)
• Length: 163 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJO48_RS08115 (MJO48_08115)	1810402..1812552	-	2151	WP_049854965.1	formate dehydrogenase subunit alpha	-
MJO48_RS08120 (MJO48_08120)	1812587..1813129	-	543	WP_013317225.1	4Fe-4S dicluster domain-containing protein	-
MJO48_RS08125 (MJO48_08125)	1813627..1813884	+	258	WP_107759008.1	DUF4160 domain-containing protein	Toxin
MJO48_RS08130 (MJO48_08130)	1813892..1814380	+	489	WP_038918520.1	addiction module antitoxin	Antitoxin
MJO48_RS08135 (MJO48_08135)	1814464..1814961	-	498	WP_038918521.1	hydrogenase maturation peptidase HycI	-
MJO48_RS08140 (MJO48_08140)	1814954..1815352	-	399	WP_049854964.1	formate hydrogenlyase maturation HycH family protein	-
MJO48_RS08145 (MJO48_08145)	1815349..1816140	-	792	WP_239788866.1	NADH-quinone oxidoreductase subunit B family protein	-
MJO48_RS08150 (MJO48_08150)	1816137..1816706	-	570	WP_039691199.1	hydrogenase 4 subunit H	-
MJO48_RS08155 (MJO48_08155)	1816717..1818444	-	1728	WP_038660219.1	hydrogenase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Uncharacterized protein HI1420 (79-157)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9995.55 Da
• Isoelectric Point: 8.1489

>T236178 WP_107759008.1 NZ_CP092458:1813627-1813884 [Dickeya fangzhongdai]
MPEIDRLAGLIFHLYFYDDERHHLPHVHVCYGNHELIISIRTGTEIEGYLPNNKRKMALAHIRAHREQLLSMWERAVVGR
NPGKL

Nucleotide

• Length: 258 bp

>T236178 NZ_LR135476:c243544-243272 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 163 a.a.
• Molecular weight: 17940.51 Da
• Isoelectric Point: 6.9795

>AT236178 WP_038918520.1 NZ_CP092458:1813892-1814380 [Dickeya fangzhongdai]
MHKIVEVDVIGDFSLRLTYEDGLVCDINLYQYASSPVFSNASLFIKFGLLPDGSLEWSPDIKISASELRKKGEYVSHTLA
VREKNFADVISRAFYDAVIENRPEILQAALKIAVEKHGNSTVAKEAGAKSRTSLYKSLNKNTHVSLATVVSLAHTVLQLE
EK

Nucleotide

• Length: 489 bp

>AT236178 NZ_LR135476:c243807-243544 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8QKC0