Detailed information of TA system TA236176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1123556..1124334
• Replicon: chromosome
• Accession: NZ_CP092458
• Organism: Dickeya fangzhongdai strain 643b

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: MJO48_RS05180
• Protein ID: WP_239788651.1
• Coordinates: 1123846..1124334 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: MJO48_RS05175
• Protein ID: WP_048331457.1
• Coordinates: 1123556..1123849 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJO48_RS05130 (MJO48_05130)	1118927..1119733	+	807	WP_239788648.1	oxidoreductase	-
MJO48_RS05135 (MJO48_05135)	1119972..1120339	+	368	Protein_1001	GNAT family N-acetyltransferase	-
MJO48_RS05140 (MJO48_05140)	1120577..1121719	+	1143	WP_107758784.1	Fic family protein	-
MJO48_RS05145 (MJO48_05145)	1121742..1121852	-	111	Protein_1003	IS3 family transposase	-
MJO48_RS05150 (MJO48_05150)	1121934..1122056	-	123	Protein_1004	hypothetical protein	-
MJO48_RS05155 (MJO48_05155)	1122037..1122255	-	219	WP_039691668.1	type II toxin-antitoxin system antitoxin CcdA	-
MJO48_RS05160 (MJO48_05160)	1122270..1122518	-	249	WP_239788649.1	helix-turn-helix transcriptional regulator	-
MJO48_RS05165 (MJO48_05165)	1122603..1122857	-	255	WP_039691665.1	helix-turn-helix transcriptional regulator	-
MJO48_RS05170 (MJO48_05170)	1122963..1123205	+	243	WP_239788650.1	helix-turn-helix transcriptional regulator	-
MJO48_RS05175 (MJO48_05175)	1123556..1123849	+	294	WP_048331457.1	DUF1778 domain-containing protein	Antitoxin
MJO48_RS05180 (MJO48_05180)	1123846..1124334	+	489	WP_239788651.1	GNAT family N-acetyltransferase	Toxin
MJO48_RS05185 (MJO48_05185)	1124469..1124670	-	202	Protein_1011	IS481 family transposase	-
MJO48_RS05190 (MJO48_05190)	1124680..1126254	-	1575	WP_239788652.1	hypothetical protein	-
MJO48_RS05195 (MJO48_05195)	1126986..1127852	+	867	WP_239788653.1	hypothetical protein	-
MJO48_RS05200 (MJO48_05200)	1127845..1128234	+	390	WP_120357961.1	hypothetical protein	-
MJO48_RS05205 (MJO48_05205)	1128355..1129098	-	744	WP_239788425.1	MobC family replication-relaxation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-95)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17469.33 Da
• Isoelectric Point: 8.7384

>T236176 WP_239788651.1 NZ_CP092458:1123846-1124334 [Dickeya fangzhongdai]
MISAPEPLRAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARKFYLRVGFEPSPIDPVMLMVTLGDLVG
SL

Nucleotide

• Length: 489 bp

>T236176 NZ_LR135475:c460804-460538 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10856.44 Da
• Isoelectric Point: 10.1968

>AT236176 WP_048331457.1 NZ_CP092458:1123556-1123849 [Dickeya fangzhongdai]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALLDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT236176 NZ_LR135475:c461067-460804 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA