Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 51847..52448 Replicon plasmid pTKEC21-59-tetX-86kb
Accession NZ_CP092453
Organism Escherichia coli isolate TKEC21-59

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag MJJ11_RS22750 Protein ID WP_001216034.1
Coordinates 52068..52448 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag MJJ11_RS22745 Protein ID WP_001190712.1
Coordinates 51847..52068 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJJ11_RS22730 (MJJ11_22730) 47667..48800 - 1134 WP_239812965.1 restriction endonuclease subunit S -
MJJ11_RS22735 (MJJ11_22735) 48920..49900 + 981 WP_000019448.1 IS5-like element ISKpn26 family transposase -
MJJ11_RS22740 (MJJ11_22740) 50108..51664 - 1557 WP_032174004.1 type I restriction-modification system subunit M -
MJJ11_RS22745 (MJJ11_22745) 51847..52068 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MJJ11_RS22750 (MJJ11_22750) 52068..52448 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MJJ11_RS22755 (MJJ11_22755) 52453..52632 + 180 WP_001513661.1 hypothetical protein -
MJJ11_RS22760 (MJJ11_22760) 52660..52938 + 279 Protein_57 pdcB -
MJJ11_RS22765 (MJJ11_22765) 52943..53356 + 414 Protein_58 integrase core domain-containing protein -
MJJ11_RS22770 (MJJ11_22770) 53581..54441 - 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
MJJ11_RS22775 (MJJ11_22775) 54624..55181 - 558 WP_001235713.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid dfrA12 / aadA2 / sul3 / blaTEM-1B / qnrS1 / tet(A) / sul2 / tet(X4) / floR - 1..86914 86914
- inside IScluster/Tn blaTEM-1B / qnrS1 / tet(A) / sul2 / tet(X4) / floR - 48920..86822 37902


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T236171 WP_001216034.1 NZ_CP092453:52068-52448 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T236171 NZ_LR135446:36071-36328 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT236171 WP_001190712.1 NZ_CP092453:51847-52068 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT236171 NZ_LR135446:35809-36078 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References