Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4159325..4159822 Replicon chromosome
Accession NZ_CP092452
Organism Escherichia coli isolate TKEC21-59

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MJJ11_RS20300 Protein ID WP_001173343.1
Coordinates 4159325..4159498 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MJJ11_RS20305 Protein ID WP_000398619.1
Coordinates 4159526..4159822 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJJ11_RS20290 (4155995) 4155995..4157452 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
MJJ11_RS20295 (4157689) 4157689..4159206 + 1518 WP_001295087.1 lysine--tRNA ligase -
MJJ11_RS20300 (4159325) 4159325..4159498 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MJJ11_RS20305 (4159526) 4159526..4159822 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MJJ11_RS20310 (4160049) 4160049..4160321 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MJJ11_RS20315 (4160333) 4160333..4160563 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MJJ11_RS20320 (4160744) 4160744..4162375 + 1632 WP_001216477.1 sensor histidine kinase -
MJJ11_RS20325 (4162372) 4162372..4163091 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T236170 WP_001173343.1 NZ_CP092452:c4159498-4159325 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T236170 NZ_LR135445:c47636-47379 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT236170 WP_000398619.1 NZ_CP092452:c4159822-4159526 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT236170 NZ_LR135445:c47898-47629 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References