Detailed information of TA system TA236164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2400197..2400835
• Replicon: chromosome
• Accession: NZ_CP092452
• Organism: Escherichia coli isolate TKEC21-59

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: MJJ11_RS11625
• Protein ID: WP_000813794.1
• Coordinates: 2400659..2400835 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MJJ11_RS11620
• Protein ID: WP_001270286.1
• Coordinates: 2400197..2400613 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ11_RS11600 (2395349)	2395349..2396290	-	942	WP_001251313.1	ABC transporter permease	-
MJJ11_RS11605 (2396291)	2396291..2397304	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
MJJ11_RS11610 (2397322)	2397322..2398467	-	1146	WP_000047421.1	ABC transporter substrate-binding protein	-
MJJ11_RS11615 (2398712)	2398712..2400118	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
MJJ11_RS11620 (2400197)	2400197..2400613	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MJJ11_RS11625 (2400659)	2400659..2400835	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MJJ11_RS11630 (2401057)	2401057..2401287	+	231	WP_000494244.1	YncJ family protein	-
MJJ11_RS11635 (2401379)	2401379..2403340	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MJJ11_RS11640 (2403413)	2403413..2403949	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
MJJ11_RS11645 (2404041)	2404041..2405216	+	1176	WP_239812881.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2400197..2416750	16553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T236164 WP_000813794.1 NZ_CP092452:c2400835-2400659 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T236164 NZ_LR135436:c129879-129607 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT236164 WP_001270286.1 NZ_CP092452:c2400613-2400197 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT236164 NZ_LR135436:c130142-129879 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• PDB: 6HPB