Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 28928..29529 Replicon plasmid pTKEC21-17-tetX-91kb
Accession NZ_CP092450
Organism Escherichia coli isolate TKEC21-17

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag MJJ13_RS22620 Protein ID WP_001216034.1
Coordinates 28928..29308 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag MJJ13_RS22625 Protein ID WP_001190712.1
Coordinates 29308..29529 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJJ13_RS22595 (MJJ13_22595) 26195..26752 + 558 WP_001235713.1 recombinase family protein -
MJJ13_RS22600 (MJJ13_22600) 26935..27795 + 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
MJJ13_RS22605 (MJJ13_22605) 28020..28433 - 414 Protein_26 integrase core domain-containing protein -
MJJ13_RS22610 (MJJ13_22610) 28438..28716 - 279 Protein_27 pdcB -
MJJ13_RS22615 (MJJ13_22615) 28744..28923 - 180 WP_001513661.1 hypothetical protein -
MJJ13_RS22620 (MJJ13_22620) 28928..29308 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MJJ13_RS22625 (MJJ13_22625) 29308..29529 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MJJ13_RS22630 (MJJ13_22630) 29712..31268 + 1557 WP_032174004.1 type I restriction-modification system subunit M -
MJJ13_RS22635 (MJJ13_22635) 31476..32456 - 981 WP_000019448.1 IS5-like element ISKpn26 family transposase -
MJJ13_RS22640 (MJJ13_22640) 32576..33721 + 1146 WP_239816680.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(X4) / sul2 / tet(A) / qnrS1 / blaTEM-1B / sul3 / aadA2 / dfrA12 / floR - 1..91489 91489
- inside IScluster/Tn tet(X4) / sul2 / tet(A) / qnrS1 / blaTEM-1B - 3223..32456 29233


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T236148 WP_001216034.1 NZ_CP092450:c29308-28928 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T236148 NZ_LR135408:198213-198479 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT236148 WP_001190712.1 NZ_CP092450:c29529-29308 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT236148 NZ_LR135408:197950-198213 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References