Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4160077..4160574 Replicon chromosome
Accession NZ_CP092449
Organism Escherichia coli isolate TKEC21-17

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MJJ13_RS20305 Protein ID WP_001173343.1
Coordinates 4160077..4160250 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MJJ13_RS20310 Protein ID WP_000398619.1
Coordinates 4160278..4160574 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJJ13_RS20295 (4156747) 4156747..4158204 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
MJJ13_RS20300 (4158441) 4158441..4159958 + 1518 WP_001295087.1 lysine--tRNA ligase -
MJJ13_RS20305 (4160077) 4160077..4160250 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MJJ13_RS20310 (4160278) 4160278..4160574 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MJJ13_RS20315 (4160801) 4160801..4161073 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MJJ13_RS20320 (4161085) 4161085..4161315 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MJJ13_RS20325 (4161496) 4161496..4163127 + 1632 WP_001216477.1 sensor histidine kinase -
MJJ13_RS20330 (4163124) 4163124..4163843 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T236147 WP_001173343.1 NZ_CP092449:c4160250-4160077 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T236147 NZ_LR135403:c53553-52690 [Enterococcus faecium]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCGAATCGCATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGAACAGGACGAACAACAGACGTTCCCATTCAAACAGCCACAATGCTTCAAT
CTAAAGGTTATGAAACAAAAATATACGTCATGGCAGTACCGAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCTACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT236147 WP_000398619.1 NZ_CP092449:c4160574-4160278 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT236147 NZ_LR135403:c53827-53555 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References