Detailed information of TA system TA236146

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3967163..3967995
• Replicon: chromosome
• Accession: NZ_CP092449
• Organism: Escherichia coli isolate TKEC21-17

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: MJJ13_RS19305
• Protein ID: WP_239812913.1
• Coordinates: 3967163..3967537 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: MJJ13_RS19310
• Protein ID: WP_001295723.1
• Coordinates: 3967627..3967995 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ13_RS19265 (3962362)	3962362..3963468	+	1107	WP_001315214.1	N-acetylneuraminate epimerase	-
MJJ13_RS19270 (3963533)	3963533..3964513	+	981	WP_239812911.1	sialate O-acetylesterase	-
MJJ13_RS19275 (3964521)	3964521..3965171	-	651	WP_239812912.1	HNH endonuclease	-
MJJ13_RS19280 (3965308)	3965308..3965451	+	144	Protein_3793	HNH endonuclease	-
MJJ13_RS19285 (3965550)	3965550..3965735	+	186	WP_000066585.1	hypothetical protein	-
MJJ13_RS19290 (3966227)	3966227..3966376	-	150	Protein_3795	hypothetical protein	-
MJJ13_RS19295 (3966482)	3966482..3966658	-	177	WP_000839290.1	DUF957 domain-containing protein	-
MJJ13_RS19300 (3966675)	3966675..3967166	-	492	WP_000976842.1	DUF5983 family protein	-
MJJ13_RS19305 (3967163)	3967163..3967537	-	375	WP_239812913.1	TA system toxin CbtA family protein	Toxin
MJJ13_RS19310 (3967627)	3967627..3967995	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MJJ13_RS19315 (3968158)	3968158..3968379	-	222	WP_000692345.1	DUF987 domain-containing protein	-
MJJ13_RS19320 (3968448)	3968448..3968924	-	477	WP_032263694.1	RadC family protein	-
MJJ13_RS19325 (3968940)	3968940..3969413	-	474	WP_053895489.1	antirestriction protein	-
MJJ13_RS19330 (3969755)	3969755..3970573	-	819	WP_001234651.1	DUF932 domain-containing protein	-
MJJ13_RS19335 (3970726)	3970726..3970884	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	3957719..3982398	24679

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13985.01 Da
• Isoelectric Point: 7.2909

>T236146 WP_239812913.1 NZ_CP092449:c3967537-3967163 [Escherichia coli]
MKTLPDTHVRELSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T236146 NZ_LR135402:45704-46054 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT236146 WP_001295723.1 NZ_CP092449:c3967995-3967627 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT236146 NZ_LR135402:45447-45710 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Antitoxin

• AlphaFold DB: S1NM52