Detailed information of TA system TA236142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3019587..3020292
• Replicon: chromosome
• Accession: NZ_CP092449
• Organism: Escherichia coli isolate TKEC21-17

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: MJJ13_RS14710
• Protein ID: WP_000539521.1
• Coordinates: 3019587..3019973 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MJJ13_RS14715
• Protein ID: WP_001280945.1
• Coordinates: 3019963..3020292 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ13_RS14690 (3015591)	3015591..3016217	+	627	WP_001295292.1	glutathione S-transferase GstB	-
MJJ13_RS14695 (3016214)	3016214..3017329	-	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
MJJ13_RS14700 (3017440)	3017440..3017823	-	384	WP_000497137.1	biofilm formation regulator BssR	-
MJJ13_RS14705 (3018036)	3018036..3019361	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
MJJ13_RS14710 (3019587)	3019587..3019973	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJJ13_RS14715 (3019963)	3019963..3020292	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
MJJ13_RS14720 (3020362)	3020362..3021690	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
MJJ13_RS14725 (3021698)	3021698..3024046	-	2349	WP_085448431.1	EAL domain-containing protein	-
MJJ13_RS14730 (3024224)	3024224..3025135	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T236142 WP_000539521.1 NZ_CP092449:3019587-3019973 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T236142 NZ_LR135395:45704-46054 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT236142 WP_001280945.1 NZ_CP092449:3019963-3020292 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT236142 NZ_LR135395:45447-45710 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6