Detailed information of TA system TA236141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2400973..2401611
• Replicon: chromosome
• Accession: NZ_CP092449
• Organism: Escherichia coli isolate TKEC21-17

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: MJJ13_RS11635
• Protein ID: WP_000813794.1
• Coordinates: 2401435..2401611 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MJJ13_RS11630
• Protein ID: WP_001270286.1
• Coordinates: 2400973..2401389 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJJ13_RS11610 (2396125)	2396125..2397066	-	942	WP_001251313.1	ABC transporter permease	-
MJJ13_RS11615 (2397067)	2397067..2398080	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
MJJ13_RS11620 (2398098)	2398098..2399243	-	1146	WP_000047421.1	ABC transporter substrate-binding protein	-
MJJ13_RS11625 (2399488)	2399488..2400894	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
MJJ13_RS11630 (2400973)	2400973..2401389	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MJJ13_RS11635 (2401435)	2401435..2401611	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MJJ13_RS11640 (2401833)	2401833..2402063	+	231	WP_000494244.1	YncJ family protein	-
MJJ13_RS11645 (2402155)	2402155..2404116	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MJJ13_RS11650 (2404189)	2404189..2404725	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
MJJ13_RS11655 (2404817)	2404817..2405992	+	1176	WP_239812881.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2400973..2417526	16553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T236141 WP_000813794.1 NZ_CP092449:c2401611-2401435 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T236141 NZ_LR135394:487640-487981 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT236141 WP_001270286.1 NZ_CP092449:c2401389-2400973 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT236141 NZ_LR135394:487410-487640 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• PDB: 6HPB