Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1684237..1684853 Replicon chromosome
Accession NC_012207
Organism Mycobacterium bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag JTY_RS07940 Protein ID WP_003407593.1
Coordinates 1684536..1684853 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7U4BU54
Locus tag JTY_RS07935 Protein ID WP_011799188.1
Coordinates 1684237..1684539 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTY_RS07925 1680123..1681970 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
JTY_RS07930 1681971..1684223 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
JTY_RS07935 1684237..1684539 + 303 WP_011799188.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
JTY_RS07940 1684536..1684853 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
JTY_RS07945 1684850..1685854 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
JTY_RS07950 1685907..1687196 + 1290 WP_003407599.1 serine hydrolase -
JTY_RS07955 1687269..1688042 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
JTY_RS07960 1688100..1688291 - 192 WP_003407609.1 dodecin family protein -
JTY_RS22230 1688322..1688546 - 225 WP_160522526.1 NUDIX domain-containing protein -
JTY_RS07970 1688816..1689844 + 1029 WP_011799189.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T23613 WP_003407593.1 NC_012207:1684536-1684853 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T23613 NC_012207:1684536-1684853 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11199.50 Da        Isoelectric Point: 9.5564

>AT23613 WP_011799188.1 NC_012207:1684237-1684539 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VPFLVALSGIILGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT23613 NC_012207:1684237-1684539 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTTGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BU54

References