Detailed information of TA system TA236116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1296614..1297531
• Replicon: chromosome
• Accession: NZ_CP092446
• Organism: Bacillus velezensis strain R-71003

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: MJE83_RS06920
• Protein ID: WP_032866111.1
• Coordinates: 1296785..1297531 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: MJE83_RS06915
• Protein ID: WP_003154807.1
• Coordinates: 1296614..1296784 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJE83_RS06875 (1291826)	1291826..1293415	+	1590	WP_205164551.1	hypothetical protein	-
MJE83_RS06880 (1293428)	1293428..1293853	+	426	WP_050498185.1	hypothetical protein	-
MJE83_RS06885 (1293858)	1293858..1294055	+	198	WP_007610833.1	XkdX family protein	-
MJE83_RS06890 (1294112)	1294112..1294873	+	762	WP_020955694.1	hypothetical protein	-
MJE83_RS06895 (1294925)	1294925..1295188	+	264	WP_003154815.1	hemolysin XhlA family protein	-
MJE83_RS06900 (1295202)	1295202..1295465	+	264	WP_003154813.1	phage holin	-
MJE83_RS06905 (1295479)	1295479..1296357	+	879	WP_020955695.1	N-acetylmuramoyl-L-alanine amidase	-
MJE83_RS06915 (1296614)	1296614..1296784	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
MJE83_RS06920 (1296785)	1296785..1297531	-	747	WP_032866111.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MJE83_RS06925 (1297635)	1297635..1298633	-	999	WP_020955696.1	inorganic phosphate transporter	-
MJE83_RS06930 (1298646)	1298646..1299263	-	618	WP_003154804.1	DUF47 domain-containing protein	-
MJE83_RS06935 (1299549)	1299549..1300865	-	1317	WP_007407254.1	amino acid permease	-
MJE83_RS06940 (1301188)	1301188..1302138	+	951	WP_218791607.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1261610..1305788	44178

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29095.60 Da
• Isoelectric Point: 4.7755

>T236116 WP_032866111.1 NZ_CP092446:c1297531-1296785 [Bacillus velezensis]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T236116 NZ_LR135345:45704-46054 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT236116 WP_003154807.1 NZ_CP092446:c1296784-1296614 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT236116 NZ_LR135345:45447-45710 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14