Detailed information of TA system TA236115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 485356..485993
• Replicon: chromosome
• Accession: NZ_CP092446
• Organism: Bacillus velezensis strain R-71003

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: MJE83_RS02490
• Protein ID: WP_003156187.1
• Coordinates: 485643..485993 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: MJE83_RS02485
• Protein ID: WP_003156188.1
• Coordinates: 485356..485637 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJE83_RS02465 (481721)	481721..482320	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
MJE83_RS02470 (482413)	482413..482778	+	366	WP_007410229.1	holo-ACP synthase	-
MJE83_RS02475 (482943)	482943..483950	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
MJE83_RS02480 (484067)	484067..485236	+	1170	WP_025284272.1	alanine racemase	-
MJE83_RS02485 (485356)	485356..485637	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
MJE83_RS02490 (485643)	485643..485993	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MJE83_RS02495 (486111)	486111..486932	+	822	WP_003156182.1	STAS domain-containing protein	-
MJE83_RS02500 (486937)	486937..487302	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
MJE83_RS02505 (487305)	487305..487706	+	402	WP_003156178.1	anti-sigma regulatory factor	-
MJE83_RS02510 (487718)	487718..488725	+	1008	WP_052827076.1	PP2C family protein-serine/threonine phosphatase	-
MJE83_RS02515 (488789)	488789..489118	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
MJE83_RS02520 (489115)	489115..489597	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
MJE83_RS02525 (489563)	489563..490351	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
MJE83_RS02530 (490351)	490351..490953	+	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T236115 WP_003156187.1 NZ_CP092446:485643-485993 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T236115 NZ_LR135344:498986-499327 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT236115 WP_003156188.1 NZ_CP092446:485356-485637 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT236115 NZ_LR135344:498756-498986 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5