Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3641236..3641856 Replicon chromosome
Accession NZ_CP092442
Organism Citrobacter portucalensis strain Sb-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I9P40_RS17470 Protein ID WP_002892050.1
Coordinates 3641638..3641856 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag I9P40_RS17465 Protein ID WP_003021733.1
Coordinates 3641236..3641610 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I9P40_RS17455 (I9P40_14550) 3636382..3637575 + 1194 WP_003831044.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I9P40_RS17460 (I9P40_14545) 3637598..3640747 + 3150 WP_008783964.1 efflux RND transporter permease AcrB -
I9P40_RS17465 (I9P40_14540) 3641236..3641610 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
I9P40_RS17470 (I9P40_14535) 3641638..3641856 + 219 WP_002892050.1 HHA domain-containing protein Toxin
I9P40_RS17475 (I9P40_14530) 3642038..3642589 + 552 WP_198801665.1 maltose O-acetyltransferase -
I9P40_RS17480 (I9P40_14525) 3642706..3643176 + 471 WP_198801664.1 YlaC family protein -
I9P40_RS17485 (I9P40_14520) 3643255..3643395 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
I9P40_RS17490 (I9P40_14515) 3643397..3643657 - 261 WP_008783961.1 type B 50S ribosomal protein L31 -
I9P40_RS17495 (I9P40_14510) 3643846..3645399 + 1554 WP_048214784.1 EAL domain-containing protein -
I9P40_RS17500 (I9P40_14505) 3645451..3645804 - 354 WP_008783959.1 DUF1428 family protein -
I9P40_RS17505 (I9P40_14500) 3645869..3646498 - 630 WP_032942547.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T236110 WP_002892050.1 NZ_CP092442:3641638-3641856 [Citrobacter portucalensis]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T236110 NZ_LR135339:200270-200536 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT236110 WP_003021733.1 NZ_CP092442:3641236-3641610 [Citrobacter portucalensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT236110 NZ_LR135339:200007-200270 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References