Detailed information of TA system TA236109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2472977..2473713
• Replicon: chromosome
• Accession: NZ_CP092442
• Organism: Citrobacter portucalensis strain Sb-2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: I9P40_RS11705
• Protein ID: WP_198802197.1
• Coordinates: 2473231..2473713 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: I9P40_RS11700
• Protein ID: WP_008784769.1
• Coordinates: 2472977..2473243 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9P40_RS11680 (I9P40_20320)	2468029..2469216	+	1188	WP_048235374.1	outer membrane protein transport protein	-
I9P40_RS11685 (I9P40_20315)	2469454..2470083	+	630	WP_008784772.1	winged helix-turn-helix transcriptional regulator	-
I9P40_RS11690 (I9P40_20310)	2470200..2470586	-	387	WP_008784771.1	hypothetical protein	-
I9P40_RS11695 (I9P40_20305)	2470841..2472823	+	1983	WP_198802199.1	alkyl sulfatase dimerization domain-containing protein	-
I9P40_RS11700 (I9P40_20300)	2472977..2473243	+	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
I9P40_RS11705 (I9P40_20295)	2473231..2473713	+	483	WP_198802197.1	GNAT family N-acetyltransferase	Toxin
I9P40_RS11710 (I9P40_20290)	2474010..2475197	-	1188	WP_060682211.1	outer membrane protein transport protein	-
I9P40_RS11715 (I9P40_20285)	2475290..2476405	-	1116	WP_079934033.1	DUF4056 domain-containing protein	-
I9P40_RS11720 (I9P40_20280)	2476406..2477536	-	1131	WP_048235381.1	BamA/TamA family outer membrane protein	-
I9P40_RS11725 (I9P40_20275)	2477536..2478474	-	939	WP_008784763.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (80-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17613.45 Da
• Isoelectric Point: 9.8713

>T236109 WP_198802197.1 NZ_CP092442:2473231-2473713 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRKVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHALTDEAKRFYLHHGFKASQLQERTLFLRLS

Nucleotide

• Length: 483 bp

>T236109 NZ_LR135334:8412-8669 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT236109 WP_008784769.1 NZ_CP092442:2472977-2473243 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT236109 NZ_LR135334:8150-8419 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA