236096

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1230331..1231248 Replicon chromosome
Accession NZ_CP092439
Organism Bacillus velezensis strain 504

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag MG974_RS06505 Protein ID WP_003154806.1
Coordinates 1230502..1231248 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag MG974_RS06500 Protein ID WP_003154807.1
Coordinates 1230331..1230501 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MG974_RS06460 (MG974_06460) 1225562..1227184 + 1623 WP_003154821.1 pyocin knob domain-containing protein -
MG974_RS06465 (MG974_06465) 1227197..1227568 + 372 WP_014304856.1 XkdW family protein -
MG974_RS06470 (MG974_06470) 1227574..1227771 + 198 WP_044053582.1 XkdX family protein -
MG974_RS06475 (MG974_06475) 1227828..1228589 + 762 WP_044053129.1 hypothetical protein -
MG974_RS06480 (MG974_06480) 1228641..1228904 + 264 WP_003154815.1 hemolysin XhlA family protein -
MG974_RS06485 (MG974_06485) 1228918..1229181 + 264 WP_003154813.1 phage holin -
MG974_RS06490 (MG974_06490) 1229195..1230073 + 879 WP_058905962.1 N-acetylmuramoyl-L-alanine amidase -
MG974_RS06495 (MG974_06495) 1230109..1230234 - 126 WP_003154809.1 hypothetical protein -
MG974_RS06500 (MG974_06500) 1230331..1230501 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
MG974_RS06505 (MG974_06505) 1230502..1231248 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
MG974_RS06510 (MG974_06510) 1231352..1232350 - 999 WP_003154805.1 inorganic phosphate transporter -
MG974_RS06515 (MG974_06515) 1232363..1232980 - 618 WP_003154804.1 DUF47 domain-containing protein -
MG974_RS06520 (MG974_06520) 1233266..1234582 - 1317 WP_003154801.1 amino acid permease -
MG974_RS06525 (MG974_06525) 1234906..1235856 + 951 WP_014304860.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1197505..1235856 38351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T236096 WP_003154806.1 NZ_CP092439:c1231248-1230502 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T236096 NZ_LR135311:25712-26575 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT236096 WP_003154807.1 NZ_CP092439:c1230501-1230331 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT236096 NZ_LR135311:25438-25710 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References