Detailed information of TA system TA236093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 160015..160571
• Replicon: plasmid pRSpK102b
• Accession: NZ_CP092438
• Organism: Rhizobium sp. K102

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: MHI61_RS31365
• Protein ID: WP_239793692.1
• Coordinates: 160281..160571 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MHI61_RS31360
• Protein ID: WP_239793691.1
• Coordinates: 160015..160293 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MHI61_RS31340 (MHI61_31345)	155395..156282	-	888	WP_239793687.1	sugar ABC transporter permease	-
MHI61_RS31345 (MHI61_31350)	156382..157614	-	1233	WP_239793688.1	ABC transporter substrate-binding protein	-
MHI61_RS31350 (MHI61_31355)	157673..158626	-	954	WP_239793689.1	sugar phosphate isomerase/epimerase	-
MHI61_RS31355 (MHI61_31360)	158849..159919	+	1071	WP_239793690.1	LacI family DNA-binding transcriptional regulator	-
MHI61_RS31360 (MHI61_31365)	160015..160293	+	279	WP_239793691.1	CopG family ribbon-helix-helix protein	Antitoxin
MHI61_RS31365 (MHI61_31370)	160281..160571	+	291	WP_239793692.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MHI61_RS31370 (MHI61_31375)	160770..161201	+	432	WP_239793693.1	acetyl-CoA carboxylase biotin carboxyl carrier protein subunit	-
MHI61_RS31375 (MHI61_31380)	161201..162595	+	1395	WP_239793694.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
MHI61_RS31380 (MHI61_31385)	162592..163005	+	414	WP_239793695.1	acetyl-CoA carboxylase	-
MHI61_RS31385 (MHI61_31390)	163002..163709	+	708	WP_239793696.1	5-oxoprolinase subunit PxpB	-
MHI61_RS31390 (MHI61_31395)	163706..164701	+	996	WP_239793697.1	biotin-dependent carboxyltransferase family protein	-
MHI61_RS31395 (MHI61_31400)	164723..165490	+	768	WP_239793698.1	5-oxoprolinase subunit PxpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..405132	405132

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10955.65 Da
• Isoelectric Point: 8.5684

>T236093 WP_239793692.1 NZ_CP092438:160281-160571 [Rhizobium sp. K102]
MPQVIFSPAAIRDLERLREFLRPKNPSVARRAGETILKGVSLLAAHPHMGRLIEDLPEQYREWLIDFGESGYVARYRVDG
DILTVLAVRHQKEAGF

Nucleotide

• Length: 291 bp

>T236093 NZ_LR135308:c137304-137032 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10680.87 Da
• Isoelectric Point: 4.9445

>AT236093 WP_239793691.1 NZ_CP092438:160015-160293 [Rhizobium sp. K102]
MASPTSLKLDDELKGRVQQLAEARRRSSHWIMREAIAQYVEREERREALRQETLDAWNEFEATGLHVTGAQVEKWLSTWG
TDDELSAPECHK

Nucleotide

• Length: 279 bp

>AT236093 NZ_LR135308:c137567-137304 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA