Detailed information of TA system TA236091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 7952..8574
• Replicon: plasmid pRSpK102d
• Accession: NZ_CP092437
• Organism: Rhizobium sp. K102

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MHI61_RS27585
• Protein ID: WP_239793176.1
• Coordinates: 8215..8574 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MHI61_RS27580
• Protein ID: WP_239793175.1
• Coordinates: 7952..8215 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MHI61_RS27565 (MHI61_27570)	4204..7431	-	3228	WP_239793172.1	alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase	-
MHI61_RS27570 (MHI61_27575)	7549..7755	-	207	WP_239793173.1	DUF1963 domain-containing protein	-
MHI61_RS27575 (MHI61_27580)	7791..7931	+	141	WP_239793174.1	hypothetical protein	-
MHI61_RS27580 (MHI61_27585)	7952..8215	+	264	WP_239793175.1	PbsX family transcriptional regulator	Antitoxin
MHI61_RS27585 (MHI61_27590)	8215..8574	+	360	WP_239793176.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MHI61_RS27590 (MHI61_27595)	8724..9530	+	807	WP_239793393.1	phosphotransferase	-
MHI61_RS27595 (MHI61_27600)	9635..10678	-	1044	WP_239793394.1	magnesium and cobalt transport protein CorA	-
MHI61_RS27600 (MHI61_27605)	10814..11521	-	708	WP_239793177.1	DUF4272 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..643909	643909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12861.07 Da
• Isoelectric Point: 9.5305

>T236091 WP_239793176.1 NZ_CP092437:8215-8574 [Rhizobium sp. K102]
MVRSNAPKRGDVFLVDLNPVVGNEMKDGHRCVVISPREINLVGLCLTVPITTGGVFTRKAGLAVNISGHKTTGVALCNQV
RALDIMERVRLKTARLVDTLDTRTTDEIVARVVSIIDPA

Nucleotide

• Length: 360 bp

>T236091 NZ_LR135300:c20315-19452 [Enterococcus faecium]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCGAATCGCATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGAACAGGACGAACAACAGACGTTCCCATTCAAACAGCCACAATGCTTCAAT
CTAAAGGTTATGAAACAAAAATATACGTCATGGCAGTACCGAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCTACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9150.58 Da
• Isoelectric Point: 8.6378

>AT236091 WP_239793175.1 NZ_CP092437:7952-8215 [Rhizobium sp. K102]
MTNTAKIRRQGGATVFSIPPALLKMLGVEVGAELTLVVSNGALVATPTRAKKRYTLAELLEGADEMSELNKQAAAWNVSP
PVGNEVS

Nucleotide

• Length: 264 bp

>AT236091 NZ_LR135300:c20589-20317 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGAATAAAGAGAAAGTGGCTTAA